Hida Hideki, Masuda Tadashi, Sato Toyohiro, Kim Tae-Sun, Misumi Sachiyo, Nishino Hitoo
Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
Neuroreport. 2007 Jan 22;18(2):179-83. doi: 10.1097/WNR.0b013e328011398e.
Pleiotrophin promotes survival of dopaminergic neurons in vitro. To investigate whether pleiotrophin promotes survival of grafted dopaminergic neurons in vivo, donor cells from ventral mesencephalon were treated with pleiotrophin (100 ng/ml) during cell preparation and grafted into striatum of hemi-Parkinson model rats. Functional recovery in methamphetamine-induced rotations was improved, and more tyrosine hydroxylase-positive cells survived in the striatum in the pleiotrophin-treated group. Pleiotrophin addition to cells just before transplantation also resulted in better functional recovery; however, no caspase-3 activation was seen during cell preparation. Interestingly, the effect of pleiotrophin on the survival was additive to that of glial-cell line-derived neutropic factor. These results revealed that pleiotrophin had effects on donor cells in neural transplantation in vivo.
多效生长因子在体外可促进多巴胺能神经元的存活。为研究多效生长因子在体内是否能促进移植的多巴胺能神经元的存活，在细胞制备过程中，用多效生长因子（100纳克/毫升）处理中脑腹侧的供体细胞，并将其移植到半帕金森模型大鼠的纹状体中。在多效生长因子处理组中，甲基苯丙胺诱导的旋转行为的功能恢复得到改善，纹状体中存活的酪氨酸羟化酶阳性细胞更多。在移植前即刻将多效生长因子添加到细胞中也导致了更好的功能恢复；然而，在细胞制备过程中未观察到半胱天冬酶-3的激活。有趣的是，多效生长因子对存活的影响与胶质细胞系源性神经营养因子的影响具有相加性。这些结果表明，多效生长因子对体内神经移植中的供体细胞有作用。
