Su Yong, Yao Wen, Zhu Wei-yun
Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China.
Wei Sheng Wu Xue Bao. 2006 Dec;46(6):961-6.
Changes of bacterial flora from hindguts of piglets from 7 to 35 days of age (two weeks after weaning) were studied after oral administration of L. amylovorus S1, using molecular techniques based on 16S rDNA gene. Six litters of neonatal piglets were divided randomly into control group and treatment group. At 7, 9, 11 days of age, piglets in treatment group received 1, 2 and 3mL preparation of S1 (5 x 10(9) CFU/mL) through oral administration, respectively. On D 7, 14, 21, 24 and 35, one piglet from each litter was slaughtered and samples of hindguts were collected for analysis. The results showed that high G + C mol% bacteria in hindguts of piglets disappeared after weaning and restored gradually two weeks later. Sequencing analysis indicated that most of these high G + C mol% bacteria blonged to Lactobacillus spp. . Statistical analysis showed that treatment with S1 had no marked effect on diversity index of predominant bacteria from hindguts in piglets. By comparing the bands in DGGE profiles between two groups, a specific band in treatment group was found in profiles from piglets at 14 days of age, sequence matched with that showed 95 % similarity to Clostridium disporicum. At 35 days of age, another specific band appeared in control group, which was identified to be Streptococcus suis (99% ).
采用基于16S rDNA基因的分子技术，研究了7至35日龄（断奶后两周）仔猪口服解淀粉乳杆菌S1后后肠细菌菌群的变化。将6窝新生仔猪随机分为对照组和处理组。在7、9、11日龄时，处理组仔猪分别口服1、2和3mL S1制剂（5×10⁹ CFU/mL）。在第7、14、21、24和35天，从每窝中宰杀1头仔猪，收集后肠样本进行分析。结果表明，仔猪断奶后后肠中高G+C mol%的细菌消失，两周后逐渐恢复。测序分析表明，这些高G+C mol%的细菌大多属于乳杆菌属。统计分析表明，S1处理对仔猪后肠优势菌的多样性指数无显著影响。通过比较两组DGGE图谱中的条带，在14日龄仔猪的处理组图谱中发现了一条特异性条带，其序列与双孢梭菌的序列相似度为95%。在35日龄时，对照组出现了另一条特异性条带，鉴定为猪链球菌（99%）。
