Stoev G, Stoyanov Al, Shopova M, Dachev Kr
Central Laboratory of Veterinary Control and Ecology, Iskarsko Shouse 5, 1528 Sofia, Bulgaria.
J Chromatogr A. 2007 Mar 23;1145(1-2):141-8. doi: 10.1016/j.chroma.2007.01.071. Epub 2007 Jan 26.
Photodit (porfimer sodium) is a complex mixture of porphyrin-modified oligomers that has demonstrated high photoactivity during photodynamic therapy of malignant tumors. Hematoporphyrin derivative monomer precursors of Photodit and its oligomers were analyzed using liquid chromatography and mass spectrometry to determine their chemical composition and structures. Hematoporphyrin (M-3), O,O'-diacetylhematoporphyrin (M-6), and isomers of 8(-3)-hydroxyethyl-3(-8)-vinyldeuteroporphyrin (M-2), 8(-3)-(1-acetoxyethyl)-3(-8)-vinyldeuteroporphyrin (M-4), and O-acetylhematoporphyrin (M-5), in ratio 0.59:0.94:0.21:0.34:1.00, were identified as hematoporphyrin derivative monomer precursors. The main compounds of Photodit as a porfimer sodium salt were dimers: D-1 [di-[8(-3)-hydroxyethyl-3(-8)-vinyldeuteroporphyrin] ether], D-2 [[Hematoporphyrin-8(-3)-hydroxyethyl-3(-8)-vinyldeutroporphyrin] ether], and D-3 [Dihematoporphyrin ether] in ratio 0.51:1.00:0.50, and trimers: T-1 [(M-2)-(D-2) ether], T-2 [(D-2)-(M-3) ether], and T-3 [(D-3)-(M-3) ether] in ratio 0.98:1.00:0.44, respectively. An average oligomer length of 2.23 porphyrin units was determined.
Photodit（卟吩姆钠）是一种卟啉修饰的低聚物的复杂混合物，在恶性肿瘤的光动力治疗中已显示出高光活性。使用液相色谱和质谱对Photodit及其低聚物的血卟啉衍生物单体前体进行了分析，以确定其化学组成和结构。血卟啉（M-3）、O,O'-二乙酰血卟啉（M-6）以及8(-3)-羟乙基-3(-8)-乙烯基去铁卟啉（M-2）、8(-3)-(1-乙酰氧基乙基)-3(-8)-乙烯基去铁卟啉（M-4）和O-乙酰血卟啉（M-5）的异构体，比例为0.59:0.94:0.21:0.34:1.00，被鉴定为血卟啉衍生物单体前体。作为卟吩姆钠盐的Photodit的主要化合物是二聚体：D-1 [二-[8(-3)-羟乙基-3(-8)-乙烯基去铁卟啉]醚]、D-2 [[血卟啉-8(-3)-羟乙基-3(-8)-乙烯基去铁卟啉]醚]和D-3 [双血卟啉醚]，比例为0.51:1.00:0.50，以及三聚体：T-1 [(M-2)-(D-2)醚]、T-2 [(D-2)-(M-3)醚]和T-3 [(D-3)-(M-3)醚]，比例分别为0.98:1.00:0.44。确定平均低聚物长度为2.23个卟啉单元。
