Musselman B, Kessel D, Chang C K
Department of Biochemistry, Michigan State University, East Lansing 48824.
Biomed Environ Mass Spectrom. 1988 Mar 1;15(5):257-63. doi: 10.1002/bms.1200150505.
Photodynamic therapy (PDT) involves the treatment of tumor tissue with a photosensitizer and light to effect the delineation and/or eradication of the tumor. PDT is a two step process: (1) incorporation of photosensitizer into the cell where it must be retained by tumors in vivo; and (2) illumination of the tumor cell with light to effect cell death. Hematoporphyrin derivative (HPD) is a complex mixture of porphyrins currently used for PDT in the clinical setting. Hematoporphyrin-based oligomers of up to five subunits were determined using fast atom bombardment mass spectrometry of the high-molecular-weight fraction of the drug. Reduction of this fraction with LiAlH4 permitted determination of the covalent bond linking the monomers in these oligoporphyrins. Application of these analytical procedures to the determination of the composition of different preparations of HPD will be described.
光动力疗法（PDT）是指用一种光敏剂和光来治疗肿瘤组织，以实现肿瘤的轮廓勾勒和/或根除。光动力疗法是一个两步过程：（1）将光敏剂导入细胞，且它必须在体内被肿瘤细胞留存；（2）用光照射肿瘤细胞以导致细胞死亡。血卟啉衍生物（HPD）是卟啉的一种复杂混合物，目前在临床环境中用于光动力疗法。通过对该药物高分子量部分进行快速原子轰击质谱分析，确定了多达五个亚基的基于血卟啉的低聚物。用LiAlH4还原该部分，从而确定这些寡聚卟啉中连接单体的共价键。将描述这些分析程序在测定不同制剂的血卟啉衍生物组成方面的应用。
