Evans C R, Pierrepoint C G
Vet Rec. 1975 Dec 13;97(24):464-7.
Mammary tumour tissue from two bitches and an anal adenoma from a dog were investigated for steroid receptor interaction. Both mammary tumours possessed cytoplasmic macromolecules sedimenting with coefficients of 4S and 8S that bound oestradiol-17beta. These receptors had molecular weights of approximately 60,000 and 180,000 respectively. Transfer of the oestrogen to the nucleus was shown and the presence of a 4-5S nuclear protein demonstrated. The anal adenoma had a cytoplasmic receptor, with a sedimentation value in a sucrose density gradient of 4-5S with respect to bovine serum albumin, that bound tritiated 5alpha-androstane-3alpha, 17alpha-diol. No affinity could be demonstrated for other C19-steroids examined. The significance of these findings in terms of the hormone dependence of the tumours investigated and the possible development of these studies to promote rational therapy in such cases is discussed.
对两只母犬的乳腺肿瘤组织和一只犬的肛门腺瘤进行了类固醇受体相互作用的研究。两个乳腺肿瘤均具有沉降系数为4S和8S的细胞质大分子，它们能结合雌二醇-17β。这些受体的分子量分别约为60,000和180,000。研究表明雌激素可转移至细胞核，并证实存在一种4 - 5S的核蛋白。肛门腺瘤有一个细胞质受体，相对于牛血清白蛋白，其在蔗糖密度梯度中的沉降值为4 - 5S，该受体能结合氚标记的5α-雄甾烷-3α,17α-二醇。对于所检测的其他C19 - 类固醇，未显示出亲和力。讨论了这些发现对于所研究肿瘤的激素依赖性的意义，以及这些研究在促进此类病例合理治疗方面可能的发展。
