Monson K R, Malbica J O
Am J Vet Res. 1977 Dec;38(12):1937-9.
Dextran-coated charcoal assay with Scatchard plotting was used for the quantitative determination of high-affinity estradiol receptors (ER) in 10 canine mammary tumor cytosols. Mammary tumors diagnosed histopathologically as adenocarcinomas appeared to have a higher incidence of ER than those diagnosed as mixed mammary tumors. These receptors have a high affinity for estradiol with dissociation constants as determined by Scatchard analysis ranging from 1.2 to 2.0 X 10(-11) M. These dissociation constants are similar to those previously reported for the receptor-estradio interaction from target tissues in other species. The specific binding of the receptor for the hormone was diminished in the presence of an antiestrogenic compound. Our data suggest that canine mammary tumors with significant ER levels would respond favorably to antiestrogen therapy. With the demonstration of ER sites in canine mammary adenocarcinomas as presented herein, the potential for chemotherapeutic management appears to be encouraging.
采用葡聚糖包被活性炭分析法及Scatchard作图法，对10例犬乳腺肿瘤细胞溶质中的高亲和力雌二醇受体（ER）进行定量测定。经组织病理学诊断为腺癌的乳腺肿瘤，其ER发生率似乎高于诊断为混合性乳腺肿瘤的病例。这些受体对雌二醇具有高亲和力，通过Scatchard分析测定的解离常数范围为1.2至2.0×10⁻¹¹ M。这些解离常数与先前报道的其他物种靶组织中受体 - 雌二醇相互作用的解离常数相似。在抗雌激素化合物存在下，受体对激素的特异性结合减少。我们的数据表明，具有显著ER水平的犬乳腺肿瘤对抗雌激素治疗将有良好反应。如本文所述，在犬乳腺腺癌中证实了ER位点，化疗管理的潜力似乎令人鼓舞。
