Programa de Pós Graduação em Ciência de Alimentos, Faculdade de Farmácia; Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Nutrition. 2010 Feb;26(2):218-23. doi: 10.1016/j.nut.2009.04.005. Epub 2009 Aug 5.
To evaluate the effects of arginine on intestinal barrier integrity and bacterial translocation (BT) in mice undergoing intestinal obstruction.
Mice were divided into 3 groups, treated for 7 d before surgical intervention with isocaloric and isoprotein diets. The ARG group received a diet containing 2% arginine, the IO (intestinal obstruction) and Sham groups, standard chow diet. On the eighth day of treatment, all animals received diethylenetriamine pentaacetic acid (DTPA) solution labeled with 99mTechnetium (99mTc-DTPA) by gavage for intestinal permeability analysis. After 90 min, the animals were anesthetized and the terminal ileum ligated. The Sham group only underwent laparotomy. After 4, 8, and 18 h, blood was collected for radioactivity determination. Samples of ileum were collected 18 h after surgery for histological analysis. In another set of animals, BT was evaluated. After 7 d of treatment, all animals received 10(8) CFU/mL of 99mTc-E.coli by gavage; 90 min later they were submitted to the surgical procedure described above. BT was determined by the uptake of 99mTc-E.coli in blood, mesenteric lymph nodes, liver, spleen, and lungs, assessed 18 h after the surgery.
The intestinal permeability and BT were higher in the IO group when compared with the Sham group (P < 0.05). Arginine supplementation reduced intestinal permeability and BT to physiologic levels. Histological analysis showed mucosal ileum preservation in animals treated with arginine.
Arginine was able to preserve barrier integrity, thus reducing BT.
评估精氨酸对肠梗阻小鼠肠屏障完整性和细菌易位（BT）的影响。
将小鼠分为 3 组，在手术干预前用等热量和等蛋白饮食进行 7 天治疗。ARG 组给予含 2%精氨酸的饮食，IO（肠梗阻）和 Sham 组给予标准饲料。治疗第 8 天，所有动物均通过灌胃接受二乙三胺五乙酸（DTPA）标记 99m 锝（99mTc-DTPA）溶液进行肠道通透性分析。90 分钟后，动物麻醉并结扎末端回肠。 Sham 组仅行剖腹术。4、8 和 18 小时后，采集血液进行放射性测定。手术后 18 小时采集回肠样本进行组织学分析。在另一组动物中，评估 BT。治疗 7 天后，所有动物通过灌胃给予 10（8）CFU/mL 的 99mTc-E.coli；90 分钟后，进行上述手术程序。通过血液、肠系膜淋巴结、肝脏、脾脏和肺部中 99mTc-E.coli 的摄取来确定 BT，在手术后 18 小时进行评估。
与 Sham 组相比，IO 组的肠道通透性和 BT 更高（P < 0.05）。精氨酸补充将肠道通透性和 BT 降低至生理水平。组织学分析显示，用精氨酸治疗的动物的回肠黏膜得到了保存。
精氨酸能够维持屏障完整性，从而减少 BT。
