Avani G, Rao M V
Department of Zoology, University School of Sciences, Gujarat University, Navrangpura, Ahmedabad 380 009, Gujarat, India.
Toxicol In Vitro. 2007 Jun;21(4):626-31. doi: 10.1016/j.tiv.2006.12.010. Epub 2007 Jan 9.
Arsenic is a ubiquitous trace element and a well-established human carcinogen. In search for an 'antidote' to this global poison, this work was undertaken to study the probable beneficial effect of vitamin A upon arsenic induced genotoxicity. Peripheral blood lymphocyte culture was carried out to study the effects of arsenic at three different dose levels (0.5, 1 and 2 microg) for 24 h prior to harvesting. In addition, mutagenic in vitro effect of ethyl methanesulphonate was studied as a positive control. Genotoxic variables presented here are sister chromatid exchanges (SCE), cell cycle proliferative index/replicative index (CCPI/RI), average generation time (AGT) and population doubling time (PDT). Inevitably, arsenic treatment showed dose-dependent augmentation in the incidences of SCE and CCPI/RI together with AGT and PDT. However, vitamin A supplemented arsenic cultures demonstrated remarkable resurgence in the described genotoxic parameters. This data shows that vitamin A might be a useful interventional treatment in arsenic poisoning.
砷是一种普遍存在的微量元素，也是一种公认的人类致癌物。为了寻找这种全球毒物的“解药”，开展了这项工作，以研究维生素A对砷诱导的遗传毒性可能产生的有益作用。在收获前24小时，进行外周血淋巴细胞培养，以研究三种不同剂量水平（0.5、1和2微克）的砷的作用。此外，研究了甲磺酸乙酯的体外诱变作用作为阳性对照。这里呈现的遗传毒性变量有姐妹染色单体交换（SCE）、细胞周期增殖指数/复制指数（CCPI/RI）、平均世代时间（AGT）和群体倍增时间（PDT）。不可避免地，砷处理显示SCE和CCPI/RI以及AGT和PDT的发生率呈剂量依赖性增加。然而，补充了维生素A的砷培养物在所描述的遗传毒性参数方面表现出显著恢复。该数据表明，维生素A可能是砷中毒的一种有用的干预治疗方法。
