Avani G, Rao M V
Department of Zoology, University School of Sciences, Gujarat University, Navrangpura, Ahmedabad 380 009, Gujarat, India.
Phytomedicine. 2008 Mar;15(3):221-5. doi: 10.1016/j.phymed.2007.03.009. Epub 2007 May 4.
In vitro effects of arsenic in human peripheral lymphocytes (HPL) at three different doses - 3.6x10(-4), 1.4 x 10(-3) and 0.72 x 10(-3) microM for 24h before harvesting on sister chromatid exchanges (SCE), Cell cycle proliferative index/replicative index (CCPI/RI), %M(1), %M(2) and %M(3), population doubling time (PDT) and average generation time (AGT) were examined. Andrographis paniculata (commonly referred to as 'kalmegh') has been used for centuries in traditional Indian and Chinese herbal medicine as a safe, natural folk remedy for assorted health concerns. In the present study, kalmegh (0.01 microg/7ml culture media) was used along with the highest dose of arsenic; the results showed that arsenic induced increase in these genotoxic endpoints were fairly diminished by kalmegh. In addition, mutagenic in vitro effect of ethyl methanesulphonate (EMS) was used as a positive control in this study. It is thus concluded from this study that Andrographis has a protective role in arsenic toxicity.
在收获前24小时，研究了三种不同剂量（3.6×10⁻⁴、1.4×10⁻³和0.72×10⁻³微摩尔）的砷对人外周血淋巴细胞（HPL）的体外作用，包括姐妹染色单体交换（SCE）、细胞周期增殖指数/复制指数（CCPI/RI）、%M₁、%M₂和%M₃、群体倍增时间（PDT）和平均世代时间（AGT）。穿心莲（通常称为“苦草”）在传统印度和中国草药中已被使用了几个世纪，作为一种安全、天然的民间疗法用于各种健康问题。在本研究中，将苦草（0.01微克/7毫升培养基）与最高剂量的砷一起使用；结果表明，苦草相当程度地减轻了砷诱导的这些遗传毒性终点的增加。此外，在本研究中使用甲磺酸乙酯（EMS）的体外诱变作用作为阳性对照。因此，本研究得出结论，穿心莲对砷毒性具有保护作用。
