用于定量测定人尿中西兰花总异硫氰酸盐的标准品的改进合成方法。

Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine.

作者信息

Kristensen Mette, Krogholm Kirstine S, Frederiksen Hanne, Duus Fritz, Cornett Claus, Bügel Susanne H, Rasmussen Salka E

机构信息

Department of Toxicology and Risk Assessment, Danish Institute for Food and Veterinary Research, 2860 Søborg, Denmark.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 1;852(1-2):229-34. doi: 10.1016/j.jchromb.2007.01.022. Epub 2007 Jan 26.

DOI:10.1016/j.jchromb.2007.01.022

PMID:17307405

Abstract

A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesis of the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-L-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1+/-25.2% of the dose.

摘要

一种用于定量异硫氰酸酯（ITC）及其代谢物的知名方法是与1,2 - 苯二硫醇发生缩合反应生成1,3 - 苯并二硫醇 - 2 - 硫酮，该产物可通过高效液相色谱法定量。此测定需要ITC代谢物和1,3 - 苯并二硫醇 - 2 - 硫酮的标准品，但市面上无售。在本研究中，我们报道了ITC代谢物N - 乙酰 - S -（N - 4 - 甲基亚磺酰丁基硫代氨基甲酰基）-L - 半胱氨酸和1,3 - 苯并二硫醇 - 2 - 硫酮的改进合成方法。这些标准品用于定量10名食用350克西兰花的健康受试者尿液中ITC的排泄量。在48小时内对排泄情况进行了研究，结果显示尿液中ITC的累积排泄量为剂量的49.1±25.2%。