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用巯基乙醇进行柱前衍生化后，通过反相液相色谱法同时测定多种异硫氰酸酯。

Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol.

作者信息

Wilson Eli Adjélé, Ennahar Saïd, Zhao Minjie, Bergaentzle Martine, Marchioni Eric, Bindler Françoise

机构信息

Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Faculté de Pharmacie 74, route du Rhin, 67400 Illkirch, France.

出版信息

Chromatographia. 2011 Jun;73(Suppl 1):137-142. doi: 10.1007/s10337-010-1878-1. Epub 2011 Jan 8.

DOI:10.1007/s10337-010-1878-1
PMID:21765536
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3098979/
Abstract

Numerous isothiocyanates (ITCs) are poorly soluble in water which causes their precipitation in aqueous mobile phases used in reversed phase liquid chromatography (RP-LC), thus impacting the accuracy of the quantification. By comparing the amounts of ITCs injected and released from the column, losses could be estimated at 5-32% depending on polarities and concentrations. Results could be dramatically improved in terms of separation and quantification using RP-LC with a mercaptoethanol precolumn derivatization aimed at avoiding ITCs precipitation. The cancer chemoprotective allyl-ITC and sulforaphane were found in cabbage extracts at 1.2 and 2.7 μg g(-1) fresh weight, respectively.

摘要

许多异硫氰酸酯（ITC）在水中的溶解度很差，这导致它们在反相液相色谱（RP-LC）的水相流动相中沉淀，从而影响定量的准确性。通过比较注入色谱柱和从色谱柱中洗脱出来的ITC的量，可以估计损失在5%-32%之间，具体取决于极性和浓度。使用带有巯基乙醇预柱衍生化的RP-LC可以显著改善分离和定量结果，以避免ITC沉淀。在卷心菜提取物中发现具有癌症化学预防作用的烯丙基-ITC和萝卜硫素，其鲜重分别为1.2和2.7μg g(-1)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fd7/3098979/81248d8cc3c9/10337_2010_1878_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fd7/3098979/f77bfcc72cc9/10337_2010_1878_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fd7/3098979/6e33b97a7075/10337_2010_1878_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fd7/3098979/81248d8cc3c9/10337_2010_1878_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fd7/3098979/f77bfcc72cc9/10337_2010_1878_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fd7/3098979/6e33b97a7075/10337_2010_1878_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fd7/3098979/81248d8cc3c9/10337_2010_1878_Fig3_HTML.jpg

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