Huang Bo, Yu Fang, Zare Richard N
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
Anal Chem. 2007 Apr 1;79(7):2979-83. doi: 10.1021/ac062284x. Epub 2007 Feb 20.
We designed, constructed, and tested a surface plasmon resonance (SPR) microscope using a high numerical aperture objective from a commercially available inverted optical microscope. Such a configuration, combined with various methods to shorten the surface plasmon propagation length, achieves diffraction-limited spatial resolution in the transverse direction and near-diffraction-limited resolution in the longitudinal direction. A virtue of the objective-type SPR imaging is that we achieve distortion-free angle-resolved SPR imaging, allowing the angle-dependent reflectivity of the sample to be examined on a pixel-by-pixel basis, thus offering high-resolution information about surface properties.
我们设计、构建并测试了一种表面等离子体共振（SPR）显微镜，它使用了市售倒置光学显微镜中的高数值孔径物镜。这种配置，再结合各种缩短表面等离子体传播长度的方法，在横向实现了衍射极限空间分辨率，在纵向实现了近衍射极限分辨率。物镜型SPR成像的一个优点是我们实现了无畸变的角度分辨SPR成像，能够逐像素地检测样品的角度相关反射率，从而提供有关表面特性的高分辨率信息。
