National & Local Joint Engineering Research Center for Mineral Salt Deep Utilization, Faculty of Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, China.
Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Biosensors (Basel). 2024 Feb 2;14(2):84. doi: 10.3390/bios14020084.
Surface plasmon resonance (SPR) is a powerful tool for determining molecular interactions quantitatively. SPR imaging (SPRi) further improves the throughput of SPR technology and provides the spatially resolved capability for observing the molecular interaction dynamics in detail. SPRi is becoming more and more popular in biological and chemical sensing and imaging. However, SPRi suffers from low spatial resolution due to the imperfect optical components and delocalized features of propagating surface plasmonic waves along the surface. Diverse kinds of approaches have been developed to improve the spatial resolution of SPRi, which have enormously impelled the development of the methodology and further extended its possible applications. In this minireview, we introduce the mechanisms for building a high-spatial-resolution SPRi system and present its experimental schemes from prism-coupled SPRi and SPR microscopy (SPRM) to surface plasmonic scattering microscopy (SPSM); summarize its exciting applications, including molecular interaction analysis, molecular imaging and profiling, tracking of single entities, and analysis of single cells; and discuss its challenges in recent decade as well as the promising future.
表面等离子体共振（SPR）是定量测定分子相互作用的有力工具。SPR 成像（SPRi）进一步提高了 SPR 技术的通量，并提供了空间分辨能力，可详细观察分子相互作用动力学。SPRi 在生物和化学传感和成像方面越来越受欢迎。然而，由于传播表面等离子体波的不完美光学元件和非局域特征，SPRi 的空间分辨率较低。已经开发了多种方法来提高 SPRi 的空间分辨率，这极大地推动了该方法的发展，并进一步扩展了其可能的应用。在这篇综述中，我们介绍了构建高空间分辨率 SPRi 系统的机制，并从棱镜耦合 SPRi 和 SPR 显微镜（SPRM）到表面等离子体散射显微镜（SPSM）展示了其实验方案；总结了它令人兴奋的应用，包括分子相互作用分析、分子成像和剖析、单分子追踪和单细胞分析；并讨论了它在最近十年的挑战以及未来的前景。
