一种水溶性八阳离子锌酞菁作为核酸二级结构的分子探针。
A water-soluble, octacationic zinc phthalocyanine as molecular probe for nucleic acid secondary structure.
作者信息
Zhang An-Ming, Huang Jing, Weng Xiao-Cheng, Li Jin-Xi, Ren Li-Ge, Song Zhi-Bin, Xiong Xiao-Qin, Zhou Xiang, Cao Xiao-Ping, Zhou Yan
机构信息
College of Chemistry and Molecular Sciences, Wuhan University, Hubei, Wuhan, PR China.
出版信息
Chem Biodivers. 2007 Feb;4(2):215-23. doi: 10.1002/cbdv.200790026.
The interaction between CT-DNA and the zinc phthalocyanine ZnPc (1) was studied by UV/VIS and fluorescence titration, as well as by thermal denaturation. ZnPc was found to strongly bind to CT-DNA (K(app)=7.35 x 10(5) M(-1)) in a non-intercalative mode. The photosensitized cleavage of pBR322 DNA was found to efficiently proceed via singlet-oxygen ((1)O(2)) production. Further, ZnPc (1) caused site-specific scission of guanine (G) bases around the bulge of the hairpin oligonucleotides OD1-OD3, as clearly shown by gel-electrophoresis experiments.
通过紫外可见光谱和荧光滴定以及热变性研究了氯化十六烷基三甲基铵（CT-DNA）与锌酞菁ZnPc（1）之间的相互作用。发现ZnPc以非嵌入模式与CT-DNA强烈结合（表观结合常数K(app)=7.35×10⁵ M⁻¹）。发现pBR322 DNA的光敏切割通过单线态氧（¹O₂）的产生有效地进行。此外，如凝胶电泳实验清楚显示的那样，ZnPc（1）导致发夹寡核苷酸OD1-OD3凸起周围的鸟嘌呤（G）碱基发生位点特异性断裂。
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