铅离子与银鲫肾脏DNA相互作用的直接证据。

Direct evidence for interaction between lead ions and kidney DNA from silver crucian carp.

作者信息

Hong Fashui, Wu Cheng, Liu Chao, Wang Ling, Gao Fengqing, Yang Fan, Xu Jianhua, Liu Tao, Xie Yaning, Li Xiangzhen

机构信息

College of Life Science, Suzhou University, Suzhou 215123, PR China.

出版信息

Chemosphere. 2007 Jul;68(8):1442-6. doi: 10.1016/j.chemosphere.2007.04.020. Epub 2007 May 22.

DOI:10.1016/j.chemosphere.2007.04.020

PMID:17517428

Abstract

A direct interaction of lead (Pb) with kidney DNA from silver crucian carp (Carassius auratus gibelio) has been systematically studied in vitro using multi-techniques, including UV-vis absorption, extended X-ray absorption fine structure, vacuum ultraviolet circular dichroism spectral methods and gel electrophoresis. We find that Pb is bound with four oxygen or nitrogen atoms of DNA in its fresh shell at the distances of 2.64 A. Additionally, Pb may bind to the oxygen atom of nucleic acid or nitrogen atom of base pairs of DNA in that obvious changes in the secondary structure of DNA are found after Pb treatment. However, this direct interaction of Pb with DNA do not cause DNA cleavage in vitro.

摘要

利用紫外可见吸收、扩展X射线吸收精细结构、真空紫外圆二色光谱方法和凝胶电泳等多种技术，在体外系统研究了铅（Pb）与银鲫（Carassius auratus gibelio）肾脏DNA的直接相互作用。我们发现，Pb在其新鲜壳层中与DNA的四个氧或氮原子结合，距离为2.64埃。此外，Pb可能与核酸的氧原子或DNA碱基对的氮原子结合，因为在Pb处理后发现DNA的二级结构有明显变化。然而，Pb与DNA的这种直接相互作用在体外不会导致DNA断裂。