Vanhove B, Bazin H
Experimental Immunology Unit, Faculty of Medicine, University of Louvain, Brussels, Belgium.
Mol Immunol. 1992 Jan;29(1):1-8. doi: 10.1016/0161-5890(92)90150-v.
Expression of Ig isotypes other than IgD together with IgM on the membranes of single B cells has been reported in different experimental models. This paper describes the co-expression of IgG2b or IgE with IgM-IgD on the surface of single B cell subpopulations from normal rats. Their expression was demonstrated with anti-IgE or IgG2b monoclonal antibodies and their F(ab')2 fragments. After pronase digestion, the re-expression of these isotypes together with IgM-IgD was observed in vitro and was inhibited by cycloheximide. These observations imply that mechanisms other than class switching may participate in the expression of membrane isotypes in vivo. The role of these membrane isotypes is still to be established, but could be important as IgG2b molecules are found on a large B cell subpopulation.
在不同的实验模型中，已经报道了单个B细胞膜上除IgD外的其他Ig同种型与IgM共同表达。本文描述了来自正常大鼠的单个B细胞亚群表面IgG2b或IgE与IgM-IgD的共表达。用抗IgE或IgG2b单克隆抗体及其F(ab')2片段证明了它们的表达。经链霉蛋白酶消化后，在体外观察到这些同种型与IgM-IgD一起重新表达，并且这种表达受到环己酰亚胺的抑制。这些观察结果表明，除类别转换外的其他机制可能参与体内膜同种型的表达。这些膜同种型的作用仍有待确定，但可能很重要，因为在一个大的B细胞亚群上发现了IgG2b分子。
