Vanhove B, Bazin H
Experimental Immunology Unit, Faculty of Medicine, University of Louvain, Brussels, Belgium.
Immunology. 1993 Aug;79(4):580-6.
Rat spleen cells were stimulated with pokeweed mitogen (PWM) and the IgM and IgE responses were assessed. An enrichment of the cell suspension with IgE-bearing cells before stimulation resulted in an increase in the number of IgE-secreting cells. A decrease of the number of IgE-secreting cells was found after depletion of IgE- or IgM-bearing cells, but not those bearing IgD molecules on their membranes, before stimulation. Moreover, the stimulation of membrane IgE on B cells with anti-IgE antibodies was shown to increase the number of IgE-secreting cells after PWM-induced differentiation in vitro. In vivo, it was also observed that a single injection of anti-IgE antibodies can induce the differentiation of IgE-secreting cells. These results demonstrate the presence of IgE(+)-IgM (+)-IgD- B cells in the rat that are responsive to PWM-induced differentiation into IgE-secreting cells. They indicate a pre-commitment of these cells at a stage where they still express IgM on their surface. IgE molecules on the cell membranes play a role in their differentiation.
用美洲商陆丝裂原（PWM）刺激大鼠脾细胞，并评估IgM和IgE反应。在刺激前用含IgE的细胞富集细胞悬液会导致IgE分泌细胞数量增加。在刺激前耗尽含IgE或IgM的细胞，但不包括膜上带有IgD分子的细胞后，发现IgE分泌细胞数量减少。此外，用抗IgE抗体刺激B细胞上的膜IgE被证明可增加体外PWM诱导分化后IgE分泌细胞的数量。在体内，还观察到单次注射抗IgE抗体可诱导IgE分泌细胞的分化。这些结果证明大鼠中存在IgE(+) - IgM(+) - IgD - B细胞，它们对PWM诱导分化为IgE分泌细胞有反应。它们表明这些细胞在仍在其表面表达IgM的阶段就已预先确定。细胞膜上的IgE分子在其分化中起作用。
