Trout James M, Santín Mónica, Fayer Ronald
Environmental Microbial Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, U.S. Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland, USA, 20705.
J Zoo Wildl Med. 2006 Jun;37(2):141-4. doi: 10.1638/05-06TYM-123005.1.
Feces and duodenal scrapings were collected from 22 coyotes (Canis latrans) killed in managed hunts in northeastern Pennsylvania. Polymerase chain reaction (PCR) methods were used to detect Giardia and Cryptosporidium spp. PCR-amplified fragments of Giardia and Cryptosporidium spp. SSU-rRNA genes were subjected to DNA sequence analysis for species/genotype determination. Seven coyotes (32%) were positive for G. duodenalis: three assemblage C, three assemblage D, and one assemblage B. Six coyotes (27%) were positive for Cryptosporidium spp. One isolate shared 99.7% homology with C. muris, whereas five others (23%) shared 100% homology with C. canis, coyote genotype. This is the first report on multiple genotypes of Giardia spp. in coyotes and on the prevalence of Cryptosporidium spp. genotypes in coyotes.
从宾夕法尼亚州东北部有组织狩猎中捕杀的22只郊狼（犬属）身上采集粪便和十二指肠刮片。采用聚合酶链反应（PCR）方法检测贾第虫属和隐孢子虫属。对贾第虫属和隐孢子虫属小亚基核糖体RNA（SSU-rRNA）基因的PCR扩增片段进行DNA序列分析以确定物种/基因型。7只郊狼（32%）十二指肠贾第虫检测呈阳性：3只为C型，3只为D型，1只为B型。6只郊狼（27%）隐孢子虫属检测呈阳性。一株分离株与鼠隐孢子虫有99.7%的同源性，而其他5株（23%）与犬隐孢子虫郊狼基因型有100%的同源性。这是关于郊狼体内贾第虫属多种基因型以及隐孢子虫属基因型流行情况的首次报告。
