Cao Shengkui, Xu Meng, Jiang Yanyan, Liu Hua, Yuan Zhongying, Sun Lei, Cao Jianping, Shen Yujuan
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China.
Chinese Center for Tropical Diseases Research, Shanghai, China.
Front Vet Sci. 2020 Jan 31;7:30. doi: 10.3389/fvets.2020.00030. eCollection 2020.
spp., spp. and microsporidia are important intestinal protozoa responsible for diarrhea in humans and other mammals. China is a major chicken-raising country, and studies on these protozoa in chickens have important public health significance. Here, we investigated the prevalence and genetic characterization of these parasites in chickens from Ezhou City, Hubei Province, China. In total, 206 stool specimens were collected from chickens in four villages of Ezhou between July 2014 and February 2015. Genomic DNA of each specimen was tested by nested PCR based on the small subunit rRNA gene, the triose phosphate isomerase gene, and the internal transcribed spacer of the rRNA gene, respectively. The public health significance of and identified in our study was evaluated via phylogenetic analysis. The infection rates were determined to be 2.43% (5/206), 8.25% (17/206), and 1.94% (4/206) for , and , respectively. One sample showed coinfection with and . Meanwhile, sequence analysis of the PCR-positive samples showed that the was was assemblage C, and was genotype D and novel genotype EZ0008. This is the first report of zoonotic assemblage C in chickens in the world, and the first report of zoonotic genotype D in chickens in China. These findings indicate new transmission dynamics and molecular epizootiology.
等孢球虫属、隐孢子虫属和微孢子虫是导致人类和其他哺乳动物腹泻的重要肠道原生动物。中国是主要的养鸡大国，对鸡体内这些原生动物的研究具有重要的公共卫生意义。在此，我们调查了中国湖北省鄂州市鸡群中这些寄生虫的流行情况和基因特征。2014年7月至2015年2月期间，共从鄂州市四个村庄的鸡群中收集了206份粪便样本。分别基于小亚基rRNA基因、磷酸丙糖异构酶基因和rRNA基因的内部转录间隔区，通过巢式PCR对每个样本的基因组DNA进行检测。通过系统发育分析评估了我们研究中鉴定出的等孢球虫属和隐孢子虫属的公共卫生意义。等孢球虫属、隐孢子虫属和微孢子虫的感染率分别确定为2.43%（5/206）、8.25%（17/206）和1.94%（4/206）。一个样本显示同时感染了等孢球虫属和隐孢子虫属。同时，对PCR阳性样本的序列分析表明，等孢球虫属为C群，隐孢子虫属为D基因型和新的EZ0008基因型。这是世界上首次关于鸡群中具有人畜共患病传播能力的等孢球虫属C群的报道，也是中国首次关于鸡群中具有人畜共患病传播能力的隐孢子虫属D基因型的报道。这些发现表明了新的传播动态和分子流行病学情况。
