Bajer Tomás, Adam Martin, Galla Lubomír, Ventura Karel
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
J Sep Sci. 2007 Jan;30(1):122-7. doi: 10.1002/jssc.200600306.
In the present paper, the following extraction techniques have been used for extracting isoflavonoids from the species Matricaria recutita, Rosmarinus officinalis, Foeniculum vulgare, and Agrimonia eupatoria L.: supercritical fluid extraction (SFE), pressurized fluid extraction, matrix solid phase dispersion, ultrasonic extraction in an ultrasonic bath (USE) and by means of an ultrasonic homogeniser (HOM), extraction by means of Soxhlet apparatus (SOX), and solid phase extraction. Experimental optimization of all techniques has been carried out using a soybean flour. Subsequent analyses of the extracts were carried out by liquid chromatography with UV detection. The maximum yields of daidzein and genistein were obtained by extraction with the SOX, USE, and HOM techniques. The maximum yields of apigenin and biochanin A from herb samples were obtained by SFE.
在本论文中，采用了以下提取技术从母菊、迷迭香、小茴香和龙芽草中提取异黄酮：超临界流体萃取（SFE）、加压流体萃取、基质固相分散、在超声浴中超声萃取（USE）以及借助超声均质器萃取（HOM）、索氏提取器提取（SOX）和固相萃取。所有技术均使用大豆粉进行了实验优化。随后通过带紫外检测的液相色谱法对提取物进行分析。通过SOX、USE和HOM技术提取获得了大豆苷元和染料木黄酮的最大产量。通过SFE获得了草药样品中芹菜素和鹰嘴豆芽素A的最大产量。
