Song Jing-Zheng, Mo Shi-Fu, Yip Yue-Keung, Qiao Chun-Feng, Han Quan-Bin, Xu Hong-Xi
Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Shatin, Hong Kong, China.
J Sep Sci. 2007 Apr;30(6):819-24. doi: 10.1002/jssc.200600340.
A microwave assisted extraction (MAE) procedure was first developed for the simultaneous determination of isoflavonoids and astragalosides in Radix Astragali (RA). MAE showed the highest extraction efficiency when compared to Soxhlet, reflux, and ultrasonic extraction. It was found that flavonoid glycoside malonates were converted into their related glycosides during the prolonged conventional extraction, thus affecting the reproducibility. However, the conversion was inhibited when using MAE. After being optimized in terms of solvents, microwave power, and irradiation time, MAE was used for the simultaneous determination of isoflavonoids and astragalosides in RA with HPLC-UV-evaporative light scattering detection (ELSD). Our results indicated that extraction by MAE was more effective than by other conventional techniques. Moreover, the MAE method followed by HPLC-UV-ELSD determination was a simple, rapid, and reliable method for the quality assessment of RA.
首次开发了一种微波辅助萃取（MAE）方法，用于同时测定黄芪中的异黄酮和黄芪皂苷。与索氏提取、回流提取和超声提取相比，MAE的提取效率最高。研究发现，在长时间的传统提取过程中，黄酮苷丙二酸酯会转化为其相关的糖苷，从而影响重现性。然而，使用MAE时这种转化受到抑制。在对溶剂、微波功率和辐照时间进行优化后，MAE与高效液相色谱-紫外-蒸发光散射检测（ELSD）联用，用于同时测定黄芪中的异黄酮和黄芪皂苷。我们的结果表明，MAE提取比其他传统技术更有效。此外，MAE方法结合HPLC-UV-ELSD测定是一种用于黄芪质量评估的简单、快速且可靠的方法。
