Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, M. Strzody 7, 44-100 Gliwice, Poland.
Department of Organic and Bioorganic Chemistry and Biotechnology, Faculty of Chemistry, Silesian University of Technology, Krzywoustego 4, 44-100 Gliwice, Poland.
Talanta. 2017 Jun 1;168:329-335. doi: 10.1016/j.talanta.2017.02.065. Epub 2017 Mar 1.
Natural deep eutectic solvents (NADESs) are considered to be new, safe solvents in green chemistry that can be widely used in many chemical processes such as extraction or synthesis. In this study, a simple extraction method based on NADES was used for the isolation of isoflavones (daidzin, genistin, genistein, daidzein) from soy products. Seventeen different NADES systems each including two or three components were tested. Multivariate data analysis revealed that NADES based on a 30% solution of choline chloride: citric acid (molar ratio of 1:1) are the most effective systems for the extraction of isoflavones from soy products. After extraction, the analytes were detected and quantified using ultra-high performance liquid chromatography with ultraviolet detection (UHPLC-UV). The proposed NADES extraction procedure achieved enrichment factors up to 598 for isoflavones and the recoveries of the analytes were in the range 64.7-99.2%. The developed NADES extraction procedure and UHPLC-UV determination method was successfully applied for the analysis of isoflavones in soy-containing food samples. The obtained results indicated that new natural deep eutectic solvents could be an alternative to traditional solvents for the extraction of isoflavones and can be used as sustainable and safe extraction media for another applications.
天然深共晶溶剂（NADES）被认为是绿色化学中的新型安全溶剂，可广泛应用于许多化学过程，如提取或合成。在这项研究中，使用了一种基于 NADES 的简单提取方法，从豆制品中分离异黄酮（大豆苷、染料木苷、染料木素、大豆黄素）。测试了 17 种不同的 NADES 体系，每个体系包含两种或三种成分。多元数据分析表明，基于 30%胆碱氯化物：柠檬酸（摩尔比为 1:1）的 NADES 是从豆制品中提取异黄酮最有效的体系。提取后，使用超高效液相色谱-紫外检测（UHPLC-UV）检测和定量分析分析物。所提出的 NADES 提取程序可使异黄酮的富集因子高达 598，分析物的回收率在 64.7-99.2%范围内。该方法已成功应用于含豆制品食品样品中异黄酮的分析。研究结果表明，新型天然深共晶溶剂可以替代传统溶剂用于异黄酮的提取，并可作为另一种应用的可持续和安全的提取介质。
