Quinn Claire E, Detmar Jacqui, Casper Robert F
Division of Reproductive Sciences, Samuel Lunenfeld Research Institute, and the Fran and Lawrence Bloomberg Department of Obstetrics and Gynecology, Mount Sinai Hospital, University of Toronto, Institute of Medical Sciences, Toronto, Ontario, Canada.
Fertil Steril. 2007 Oct;88(4 Suppl):1021-8. doi: 10.1016/j.fertnstert.2006.11.157. Epub 2007 Feb 20.
To assess pinopode formation in Lif null and Hoxa10 null mice with infertility secondary to failed implantation.
Controlled animal experiment.
Animal research and laboratory facility.
ANIMAL(S): Lif null, Hoxa10 null, and ICR mice and Sprague-Dawley rats.
INTERVENTION(S): Endometrial tissue was collected during the peri-implantation period and after ovariectomy.
MAIN OUTCOME MEASURE(S): Endometrial epithelial tissue was examined under scanning-electron microscopy and assigned a score depending on the number of pinopodes present.
RESULT(S): Pinopode scores in ICR, Lif null, and Hoxa10 null mice were comparable throughout the peri-implantation period, rising on day 3.5 of pregnancy and remaining elevated through to day 7.5, suggesting that pinopodes are not a good indicator of receptivity in mice. In contrast, pinopode scores in rats clearly demarcated the window of receptivity, appearing on day 4 of pregnancy and declining sharply on day 6. Pinopode scores were low in E(2)-treated ovariectomized mice, but unexpectedly, pinopode scores in vehicle-injected ovariectomized ICR mice were markedly elevated.
CONCLUSION(S): Lif null and Hoxa10 null mice, in which implantation is impaired, have a similar number of pinopodes to fertile ICR mice. Pinopodes do not define a window of implantation in mice.
评估Lif基因缺失和Hoxa10基因缺失且因着床失败导致不孕的小鼠的微绒毛形成情况。
对照动物实验。
动物研究和实验室设施。
Lif基因缺失、Hoxa10基因缺失的ICR小鼠以及Sprague-Dawley大鼠。
在着床期和卵巢切除术后收集子宫内膜组织。
在扫描电子显微镜下检查子宫内膜上皮组织，并根据微绒毛的数量给予评分。
在整个着床期，ICR小鼠、Lif基因缺失小鼠和Hoxa10基因缺失小鼠的微绒毛评分相当，在妊娠第3.5天升高，并一直维持到第7.5天，这表明微绒毛并非小鼠接受性的良好指标。相比之下，大鼠的微绒毛评分明确划分出了接受期窗口，在妊娠第4天出现，并在第6天急剧下降。在接受雌激素治疗的去卵巢小鼠中微绒毛评分较低，但出乎意料的是，注射赋形剂的去卵巢ICR小鼠的微绒毛评分明显升高。
着床受损的Lif基因缺失和Hoxa10基因缺失小鼠的微绒毛数量与可育的ICR小鼠相似。微绒毛并不能界定小鼠的着床窗口。
