Wei Zimin, Xi Beidou, Zhao Yue, Wang Shiping, Liu Hongliang, Jiang Youhai
Life Science College, Northeast Agriculture University, Harbin 150030, China.
Chemosphere. 2007 Jun;68(2):368-74. doi: 10.1016/j.chemosphere.2006.12.067. Epub 2007 Feb 20.
Municipal solid waste (MSW) compost contains a significant amount of humic substances. In this study, the compost consisted of residual MSW with the metal, plastic and glass removed. In order to enhance degradation processes and the degree of composting humification, complex microorganisms (Bacillus casei, Lactobacillus buchneri and Candida rugopelliculosa) and ligno-cellulolytic (Trichoderma and White-rot fungi) microorganisms were respectively inoculated in the composting process. During the MSW composting, humic acid (HA) was extracted and purified. Elements (C, N, H, O) and spectroscopic characteristics of the HA were determined using elementary analyzer, UV, Fourier transform infrared (FTIR), and fluorescence spectroscopy. The elements analysis, UV, FTIR and fluorescence spectra all led to the same conclusion, that is inoculations with microbes led to a greater degree of aromatization of HA than in the control process (CK) with no inoculation microbes. This indicated that inoculation with microbes in composting would improve the degree humification and maturation processes, in the following order: lingo-cellulolytic>complex microorganisms>CK. And mixed inoculation of MSW with complex microorganisms and lingo-cellulolytic during composting gave a greater degree of HA aromatization than inoculation with complex microorganisms or lingo-cellulolytic alone. But comparing with the HA of soil, the HA of MSW compost revealed a lower degree of aromatization.
城市固体废弃物（MSW）堆肥含有大量腐殖物质。在本研究中，堆肥由去除了金属、塑料和玻璃的剩余城市固体废弃物组成。为了强化降解过程和堆肥腐殖化程度，在堆肥过程中分别接种了复合微生物（干酪乳杆菌、布氏乳杆菌和皱皮假丝酵母）和木质纤维素分解菌（木霉和白腐真菌）。在城市固体废弃物堆肥过程中，提取并纯化了腐殖酸（HA）。使用元素分析仪、紫外光谱、傅里叶变换红外光谱（FTIR）和荧光光谱测定了HA的元素（C、N、H、O）和光谱特征。元素分析、紫外光谱、FTIR和荧光光谱均得出相同结论，即与未接种微生物的对照过程（CK）相比，接种微生物导致HA的芳构化程度更高。这表明在堆肥过程中接种微生物会提高腐殖化程度和成熟过程，顺序如下：木质纤维素分解菌>复合微生物>CK。并且在堆肥过程中城市固体废弃物与复合微生物和木质纤维素分解菌混合接种比单独接种复合微生物或木质纤维素分解菌产生的HA芳构化程度更高。但是与土壤中的HA相比，城市固体废弃物堆肥中的HA芳构化程度较低。
