Matter Denise, Rossano Alexandra, Limat Sandra, Vorlet-Fawer Lorianne, Brodard Isabelle, Perreten Vincent
Institute of Veterinary Bacteriology, University of Berne, Länggass-Strasse 122, Postfach, CH-3001 Bern, Switzerland.
Vet Microbiol. 2007 May 16;122(1-2):146-56. doi: 10.1016/j.vetmic.2007.01.009. Epub 2007 Jan 19.
A total of 83 Actinobacillus pleuropneumoniae and 58 Actinobacillus porcitonsillarum strains collected from slaughtered pigs in Switzerland were screened for susceptibility to 20 antimicrobial agents by MIC determinations. Resistance to sulfamethoxazole, the combination sulfamethoxazole-trimethoprim, tiamulin, tilmicosin, tetracycline, penicillin and ampicillin were found. A few A. porcitonsillarum isolates displayed decreased susceptibility to enrofloxacin. PCR analysis revealed the presence of the sul2 gene in approximately one-fifth of the sulfonamide-resistant A. pleuropneumoniae and A. porcitonsillarum isolates. The tetracycline-resistant A. pleuropneumoniae harbored tet(B) and tet(H), whereas the tetracycline-resistant A. porcitonsillarum isolates harbored the tet(B) gene. The penicillin and ampicillin-resistant A. pleuropneumoniae and A. porcitonsillarum harbored the bla(ROB-1) gene.
通过最小抑菌浓度（MIC）测定，对从瑞士屠宰猪中收集的83株胸膜肺炎放线杆菌和58株猪扁桃体放线杆菌菌株进行了20种抗菌药物的敏感性筛选。发现这些菌株对磺胺甲恶唑、磺胺甲恶唑 - 甲氧苄啶合剂、泰妙菌素、替米考星、四环素、青霉素和氨苄西林耐药。少数猪扁桃体放线杆菌分离株对恩诺沙星的敏感性降低。PCR分析显示，约五分之一对磺胺类药物耐药的胸膜肺炎放线杆菌和猪扁桃体放线杆菌分离株中存在sul2基因。对四环素耐药的胸膜肺炎放线杆菌携带tet(B)和tet(H)基因，而对四环素耐药的猪扁桃体放线杆菌分离株携带tet(B)基因。对青霉素和氨苄西林耐药的胸膜肺炎放线杆菌和猪扁桃体放线杆菌携带bla(ROB - 1)基因。
