Jalal Hamid, Al-Suwaine Abdulrahman, Stephen Hannah, Carne Christopher, Sonnex Christopher
Clinical Microbiology and Public Health Laboratory, Box 236, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QW, UK.
King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
J Med Microbiol. 2007 Mar;56(Pt 3):320-322. doi: 10.1099/jmm.0.46762-0.
This study investigated the comparative performance of the Amplicor assay and an in-house semi-automated, multiplex real-time PCR for the diagnosis of genital chlamydial infection. Four different assays, the COBAS Amplicor CT test (Amplicor PCR), in-house real-time PCR (IHRT-PCR), in-house nested cryptic plasmid PCR and in-house nested major outer membrane protein PCR, were performed on genital swabs from 1000 consecutive patients attending a genitourinary medicine clinic. The samples were designated true positive if Chlamydia trachomatis DNA was detected by at least two of the four above-mentioned assays while a sample was defined as true negative if C. trachomatis DNA was detected in only one or none of the assays. By this criterion, there were 129 true positive and 871 true negative samples for C. trachomatis DNA in this cohort. Amplicor PCR designated 144 samples positive: 128 (89%) of 144 samples were true positive and 16 (11%) were false positive. IHRT-PCR detected 126 of 129 true positive samples and did not generate any false positive results. The sensitivity of IHRT-PCR was comparable with, and specificity was higher than, Amplicor PCR for the diagnosis of genital chlamydial infection.
本研究调查了Amplicor检测法与一种内部半自动多重实时荧光定量PCR法在诊断生殖道衣原体感染方面的比较性能。对1000例连续到泌尿生殖医学门诊就诊患者的生殖道拭子进行了四种不同检测，即COBAS Amplicor CT检测（Amplicor PCR）、内部实时荧光定量PCR（IHRT-PCR）、内部巢式隐蔽质粒PCR和内部巢式主要外膜蛋白PCR。如果上述四种检测中至少两种检测到沙眼衣原体DNA，则样本被指定为真阳性；而如果仅在一种检测或没有检测中检测到沙眼衣原体DNA，则样本被定义为真阴性。按照该标准，该队列中沙眼衣原体DNA有129个真阳性样本和871个真阴性样本。Amplicor PCR将144个样本判定为阳性：144个样本中有128个（89%）为真阳性，16个（11%）为假阳性。IHRT-PCR检测到129个真阳性样本中的126个，且未产生任何假阳性结果。在诊断生殖道衣原体感染方面，IHRT-PCR的敏感性与Amplicor PCR相当，但特异性高于Amplicor PCR。
