采用检测泌尿生殖道标本α-甘露糖苷酶活性的方法快速筛查沙眼衣原体感染。
Rapid screening for Chlamydia trachomatis infection by detecting α-mannosidase activity in urogenital tract specimens.
机构信息
Department of Pathogen Biology, School of Basic Medicine, Zhengzhou University, Zhengzhou, 450001, China.
出版信息
BMC Infect Dis. 2013 Jan 24;13:36. doi: 10.1186/1471-2334-13-36.
BACKGROUND
Chlamydia trachomatis may cause multiple different urogenital tract disorders, but current non-culture assays for rapid screening of C. trachomatis typically use immunochromatography-based methods. We established another new rapid non-culture method for detection of C. trachomatis based on the measurement of α-mannosidase enzymatic activity in urogenital tract specimens.
METHOD
To evaluate the performance of this method, α-mannosidase activities of C. trachomatis serotype D strain 、 and 29 standard strains related to clinical urogenital pathogens were investigated. Furthermore, 553 urogenital tract specimens were used for clinical assays via cell culture method and ligase chain reaction method (LCR), adopting an expanded gold standard.
RESULTS
Only C. trachomatis was positive for α-mannosidase activity among different types of microbes tested in the research. When prostate fluid specimens, which have some interfering activity, were excluded, the sensitivity and specificity of the enzymatic method were 91.8% (78/85) and 98.3% (409/416), respectively. There were no significant differences (P > 0.05).
CONCLUSIONS
These results showed that α-mannosidase activity could be utilised as a screening marker of C. trachomatis infection.
背景
沙眼衣原体可引起多种不同的泌尿生殖道疾病，但目前用于快速筛查沙眼衣原体的非培养检测方法通常基于免疫层析法。我们建立了另一种基于泌尿生殖道标本中α-甘露糖苷酶酶活性检测的新型快速非培养方法来检测沙眼衣原体。
方法
为了评估该方法的性能，我们对沙眼衣原体血清型 D 株和 29 种与临床泌尿生殖道病原体相关的标准菌株的α-甘露糖苷酶活性进行了研究。此外，我们采用扩展金标准，通过细胞培养法和连接酶链反应法（LCR）对 553 例泌尿生殖道标本进行了临床检测。
结果
在研究中检测的不同类型微生物中，只有沙眼衣原体呈α-甘露糖苷酶活性阳性。当排除具有一定干扰活性的前列腺液标本时，酶法的灵敏度和特异性分别为 91.8%（78/85）和 98.3%（409/416），两者无显著差异（P>0.05）。
结论
这些结果表明，α-甘露糖苷酶活性可作为沙眼衣原体感染的筛查标志物。
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