Chen Yeechi, Munechika Keiko, Ginger David S
Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA.
Nano Lett. 2007 Mar;7(3):690-6. doi: 10.1021/nl062795z. Epub 2007 Feb 22.
We investigate the fluorescence from dyes coupled to individual DNA-functionalized metal nanoparticles. We use single-particle darkfield scattering and fluorescence microscopy to correlate the fluorescence intensity of the dyes with the localized surface plasmon resonance (LSPR) spectra of the individual metal nanoparticles to which they are attached. For each of three different dyes, we observe a strong correlation between the fluorescence intensity of the dye and the degree of spectral overlap with the plasmon resonance of the nanoparticle. On average, we observe the brightest fluorescence from dyes attached to metal nanoparticles that have a LSPR scattering peak approximately 40-120 meV higher in energy than the emission peak of the fluorophore. These results should prove useful for understanding and optimizing metal-enhanced fluorescence.
我们研究了与单个DNA功能化金属纳米颗粒偶联的染料的荧光。我们使用单粒子暗场散射和荧光显微镜，将染料的荧光强度与它们所附着的单个金属纳米颗粒的局域表面等离子体共振（LSPR）光谱相关联。对于三种不同的染料中的每一种，我们都观察到染料的荧光强度与纳米颗粒等离子体共振的光谱重叠程度之间存在很强的相关性。平均而言，我们观察到附着在金属纳米颗粒上的染料发出的荧光最亮，这些金属纳米颗粒的LSPR散射峰能量比荧光团的发射峰高约40-120 meV。这些结果对于理解和优化金属增强荧光应该是有用的。
