University of Oxford, Department of Chemistry, Physical and Theoretical Chemistry Laboratory, Oxford University, South Parks Road, Oxford, OX1 3QZ, UK.
Department of Materials, University of Oxford, Oxford, OX1 3PH, UK.
Angew Chem Int Ed Engl. 2017 Oct 2;56(41):12751-12754. doi: 10.1002/anie.201707809. Epub 2017 Sep 7.
Fluorescence microscopy and electrochemistry were employed to examine capping agent dynamics in silver nanoparticles capped with DNA intercalated with ethidium bromide, a fluorescent molecule. The capped NPs were studied first electrochemically, demonstrating that the intercalation of the capping agent promotes oxidation of the silver core, occurring at 0.50 V (vs. Ag, compared with 1.15 V for Ag NPs capped in DNA alone). Second, fluorescence electrochemical microscopy revealed that the electron transfer from the nanoparticles is gated by the capping agent, allowing dynamic insights unobservable using electrochemistry alone.
荧光显微镜和电化学被用来研究用溴化乙锭（一种荧光分子）嵌入 DNA 来包裹的银纳米粒子中盖帽剂的动态。首先对被包裹的 NPs 进行电化学研究，表明盖帽剂的嵌入促进了银核的氧化，其氧化电位为 0.50 V（相对于单独用 DNA 包裹的银纳米粒子的 1.15 V）。其次，荧光电化学显微镜揭示了从纳米粒子的电子转移受到盖帽剂的控制，这使得仅用电化学无法观察到的动态过程得以显现。
