Weissman D N, Bice D E, Muggenburg B A, Haley P J, Shopp G M, Schuyler M R
Albuquerque Veterans Administration Medical Center, NM 87108.
Am Rev Respir Dis. 1992 Jan;145(1):6-12. doi: 10.1164/ajrccm/145.1.6.
Primary immunization of the dog by intralobar instillation of particulate antigen induces an intense, localized pulmonary antibody response. In contrast, although soluble antigen can also induce local antibody responses after repeated deposition in the canine respiratory tract, its ability to induce local responses after primary immunization has not been well characterized. To document such responses, we immunized five beagle dogs using a bronchoscope to instill 10 mg keyhole limpet hemocyanin (KLH) into a single lung lobe (immunized) and saline into a contralateral lung lobe (control). Over the next 3 wk, we monitored specific immune responses in blood and bronchoalveolar lavage (BAL) fluids obtained from the immunized and control lung lobes. Primary intrapulmonary immunization of dogs with KLH resulted in anti-KLH antibody responses both in blood and in immunized and control BAL fluids. However, immunoglobulin class-specific expression of response differed between the immunized and control lung lobes. Specific IgM and IgA responses were significantly greater in the immunized lobes. In contrast, specific IgG, and cells producing specific IgG, were quantitatively similar in lavage fluids derived from immunized and control lung lobes. These studies demonstrate that primary immunization of the dog by intralobar instillation of soluble antigen stimulates a local IgM and IgA response and an IgG response that distributes to both immunized and unimmunized lung. This pattern of immunoglobulin class-specific pulmonary antibody response has the potential to importantly influence regional responses to intrapulmonary antigen.
通过叶内滴注颗粒性抗原对犬进行初次免疫可诱导强烈的局部肺部抗体反应。相比之下，尽管可溶性抗原在反复沉积于犬呼吸道后也能诱导局部抗体反应，但其在初次免疫后诱导局部反应的能力尚未得到充分表征。为了记录此类反应，我们使用支气管镜向五只比格犬的单个肺叶（免疫组）滴注10毫克钥孔戚血蓝蛋白（KLH），并向对侧肺叶（对照组）滴注生理盐水，对其进行免疫。在接下来的3周内，我们监测了从免疫组和对照组肺叶获取的血液和支气管肺泡灌洗（BAL）液中的特异性免疫反应。用KLH对犬进行肺内初次免疫后，血液以及免疫组和对照组的BAL液中均出现了抗KLH抗体反应。然而，免疫组和对照组肺叶中反应的免疫球蛋白类别特异性表达有所不同。免疫组肺叶中的特异性IgM和IgA反应明显更强。相比之下，免疫组和对照组肺叶灌洗液中特异性IgG以及产生特异性IgG的细胞在数量上相似。这些研究表明，通过叶内滴注可溶性抗原对犬进行初次免疫可刺激局部IgM和IgA反应以及分布于免疫和未免疫肺叶的IgG反应。这种免疫球蛋白类别特异性肺部抗体反应模式有可能对肺内抗原的局部反应产生重要影响。
