Bice D E, Degen M A, Harris D L, Muggenburg B A
Am Rev Respir Dis. 1982 Oct;126(4):635-9. doi: 10.1164/arrd.1982.126.4.635.
The deposition of particulate antigen into the dog lung induces a large accumulation of antigen-specific-forming cells in immunized lung lobes. The results of our previous studies with dogs indicate that these cells are recruited to the immunized lung lobes from the blood. The present study was designed to determine if the recruitment of immune cells to the lung is antigen-specific, or if changes occur in the lung after immunization that allow immune cells to enter the lung nonspecifically. Dogs were immunized in the left and right cardiac lung lobes with antigenically different particulate antigens. The number of lymphoid cells producing antibody to each antigen and the concentrations of antigen-specific antibody were measured in the immunized lung lobes and in a control lung lobe at 5 through 14 days after immunization. Similar numbers of antibody-forming cells and concentrations of antibody to both antigens were found in each immunized lung lobe, regardless of which antigen was used for immunization. These results indicate that antigen exposure alters the lung to allow a nonspecific recruitment of immune cells from the blood into the immunized lung lobes, regardless of antigen specificity. These data also provide further evidence that the antibody-forming cells found in the lung lavage fluid after localized immunization are produced in the thoracic lymph nodes or other systemic lymphoid tissues, rather than locally in lymphoid tissues in the deep lung.
将颗粒性抗原沉积到犬肺中会在免疫的肺叶中诱导大量抗原特异性形成细胞的积聚。我们之前对犬的研究结果表明，这些细胞是从血液中募集到免疫的肺叶中的。本研究旨在确定免疫细胞向肺的募集是否具有抗原特异性，或者免疫后肺中是否发生了变化从而允许免疫细胞非特异性地进入肺。用抗原性不同的颗粒性抗原对犬的左右心叶肺进行免疫。在免疫后5至14天，在免疫的肺叶和对照肺叶中测量产生针对每种抗原的抗体的淋巴细胞数量以及抗原特异性抗体的浓度。无论使用哪种抗原进行免疫，在每个免疫的肺叶中都发现了相似数量的抗体形成细胞和针对两种抗原的抗体浓度。这些结果表明，抗原暴露会改变肺，从而允许免疫细胞从血液中非特异性地募集到免疫的肺叶中，而与抗原特异性无关。这些数据还进一步证明，局部免疫后在肺灌洗液中发现的抗体形成细胞是在胸段淋巴结或其他全身淋巴组织中产生的，而不是在深部肺中的淋巴组织局部产生的。
