Bice D E, Harris D L, Hill J O, Muggenburg B A, Wolff R K
Am Rev Respir Dis. 1980 Nov;122(5):755-60. doi: 10.1164/arrd.1980.122.5.755.
The immune response in immunized and unimmunized bronchoalveolar spaces, as well as in the blood, was measured after localized deposition of antigen in the lung. Using a fiberoptic bronchoscope, groups of dogs were immunized with sheep red blood cells (SRBC) deposited into a single airway of the left apical or right apical lung lobes. Bronchoalveolar cells were obtained by lung lavage through the fiberoptic bronchoscope from the immunized lung lobe, as well as from lung lobes that did not receive antigen. Lavage cells and blood samples were collected at 3 to 21 days after immunization. The total number of lymphocytes, macrophages, and polymorphonuclear leukocytes, and the number of lymphoid cells producing anti-SRBC IgM and IgG antibodies were determined. Our results indicated that the highest number of antibody-forming cells (AFC) were found in the immunized lung lobes. An elevated, but significantly lower number of AFC were observed in the unimmunized lung lobes. The number of IgM and IgG AFC in the blood reached peak concentrations 3 to 7 days earlier than the AFC in the lung. We concluded from our data that the blood is an important source of the AFC found in the lung.
在肺部局部沉积抗原后，对免疫和未免疫的支气管肺泡腔以及血液中的免疫反应进行了测量。使用纤维支气管镜，将绵羊红细胞（SRBC）沉积到左尖叶或右尖叶肺叶的单个气道中，对犬群进行免疫。通过纤维支气管镜对免疫的肺叶以及未接受抗原的肺叶进行肺灌洗，获取支气管肺泡细胞。在免疫后3至21天收集灌洗细胞和血液样本。测定淋巴细胞、巨噬细胞和多形核白细胞的总数，以及产生抗SRBC IgM和IgG抗体的淋巴细胞数量。我们的结果表明，在免疫的肺叶中发现的抗体形成细胞（AFC）数量最多。在未免疫的肺叶中观察到AFC数量有所升高，但明显较少。血液中IgM和IgG AFC的浓度峰值比肺中的AFC提前3至7天出现。我们从数据中得出结论，血液是肺中发现的AFC的重要来源。
