Valerio Marlon Escoto, García Juan Fernández, Peinado Francisco Martín
Departamento de Edafología y Química Agrícola, Facultad de Ciencias, Universidad de Granada, Campus Fuentenueva s/n, 18002, Granada, Spain.
Sci Total Environ. 2007 May 25;378(1-2):63-6. doi: 10.1016/j.scitotenv.2007.01.007. Epub 2007 Feb 20.
In this work the different concentrations of soluble elements in soils from natural (peridotitic soils) and anthropogenic (soils affected by a pyrite-mine spill) origin, are used to determine the phytotoxicity in lettuce (Lactuca sativa L.). The solutions are obtained from soil:water extracts (1:1), having neutral pH and high concentrations of As, Pb, Zn, Mn, Co and Ni, with values exceeding the toxic level for soil solution [Bohn HL, McNeal BL, O'Connor GA. Soil Chemistry, Wiley Interscience. Wiley & Sons, New York, 1985]. The variables evaluated are: Seed Germination (SG), Root Elongation (RE), Germination Rate (GR) and Root Necrosis (RN). The most sensitive variables in the bioassay with these solutions are GR and RN, in these cases the solution causes a reduction of 44% and 67%, respectively, in relation to control (distilled water). The test using soil-water solutions is sensitive and reproducible to determine phytotoxicity in lettuce caused by potentially pollutant elements in soils.
在这项研究中，利用天然（橄榄岩土壤）和人为（受黄铁矿矿渣泄漏影响的土壤）来源土壤中不同浓度的可溶性元素，来测定生菜（Lactuca sativa L.）的植物毒性。这些溶液由土壤：水提取物（1:1）获得，具有中性pH值，且含有高浓度的砷、铅、锌、锰、钴和镍，其含量超过了土壤溶液的毒性水平[Bohn HL, McNeal BL, O'Connor GA.土壤化学，Wiley Interscience. Wiley & Sons, 纽约，1985]。所评估的变量包括：种子发芽率（SG）、根伸长率（RE）、发芽速率（GR）和根坏死率（RN）。在使用这些溶液进行的生物测定中，最敏感的变量是GR和RN，在这些情况下，与对照（蒸馏水）相比，溶液分别导致44%和67%的降低。使用土壤 - 水溶液进行的测试对于确定土壤中潜在污染元素对生菜的植物毒性具有敏感性和可重复性。
