Kasprzak Aldona, Spachacz Rafał, Hałoń Agnieszka, Jeleń Michał, Stefańska Katarzyna, Wachowiak Jacek, Trejster Ewa, Zabel Maciej
Department of Histology and Embryology, Poznań University of Medical Sciences, ul. Swiecickiego 6, 60-781 Poznań, Poland.
Med Wieku Rozwoj. 2006 Jul-Sep;10(3 Pt 1):639-48.
AIM OF the study was to analyze the cellular expression of two latent Epstein-Barr virus (EBV) proteins and one of the lytic protein, two small RNAs (EBERs) and EBV DNA in children and adult patients with B-cell non-Hodgkin's lymphomas (NHLs) and to characterise more strictly patterns of EBV latency in both groups of patients in our country.
The studies were performed on an archive tissue material from 26 children and from 27 adults with B-cell NHLs. The studies used immunocytochemical techniques, in situ hybridisation, and PCR.
The detectability and expression of EBV infection were significantly higher in children with B-cell NHLs as compared to adult patients. Expression of EBNA2 and LMP1 and that of EBERs in B-cell NHLs involved mainly cells of CD20(+) phenotype. Positive correlation was confirmed between expression of EBNA2 and LMP1 as well as between expression of the two proteins and EBERs in children with B-cell NHLs. Also in children with B-cell NHLs, type III latency form dominated. In adults the latency forms were more variable. We have demonstrated a novel form of EVB latency with no EBERs expression, noted in children as well as in adults with B-cell NHLs.
The significantly higher cellular expression and higher detectability of latent EBV infection in B-cell NHLs in children as compared to adults points to a potential role of EBV infection in the pathogenesis of lymphoma, particularly in this age group of patients.
本研究的目的是分析两种爱泼斯坦-巴尔病毒（EBV）潜伏蛋白、一种裂解蛋白、两种小RNA（EBERs）以及EBV DNA在儿童和成人B细胞非霍奇金淋巴瘤（NHL）患者中的细胞表达情况，并更严格地描述我国两组患者中EBV潜伏模式。
对26例儿童和27例成人B细胞NHL患者的存档组织材料进行研究。研究采用免疫细胞化学技术、原位杂交和聚合酶链反应。
与成人患者相比，儿童B细胞NHL患者中EBV感染的可检测性和表达显著更高。EBNA2和LMP1以及EBERs在B细胞NHL中的表达主要涉及CD20（+）表型的细胞。在儿童B细胞NHL患者中，EBNA2和LMP1的表达之间以及这两种蛋白与EBERs的表达之间证实存在正相关。同样在儿童B细胞NHL患者中，III型潜伏形式占主导。在成人中，潜伏形式更具多样性。我们在儿童和成人B细胞NHL患者中均发现了一种无EBERs表达的新型EBV潜伏形式。
与成人相比，儿童B细胞NHL患者中潜伏性EBV感染的细胞表达显著更高且可检测性更高，这表明EBV感染在淋巴瘤发病机制中具有潜在作用，尤其是在该年龄组患者中。
