Needleman B W, Wigley F M, Stair R W
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201.
Arthritis Rheum. 1992 Jan;35(1):67-72. doi: 10.1002/art.1780350111.
To determine whether interleukin-1 alpha (IL-1 alpha), IL-1 beta, IL-2, IL-4, interferon-gamma (IFN gamma), IL-6, and tumor necrosis factor alpha (TNF alpha) are detected more frequently in sera from scleroderma patients than in sera from controls.
Serum concentrations of these cytokines were measured in 78 scleroderma patients and 73 controls, using enzyme-linked immunosorbent assay, radioimmunoassay, and bioassay techniques.
IL-2, IL-4, and IL-6 were each detected more frequently in sera from scleroderma patients than in sera from controls. TNF alpha and IL-1 alpha were found with equal frequency in patient and control sera. IL-1 beta and IFN gamma were not detected in any sera.
IL-2, IL-4, and IL-6 may be among the cytokines that contribute to the disease process in scleroderma patients. To our knowledge, this is the first report of elevated serum IL-4 levels in human disease.
确定与对照组血清相比，硬皮病患者血清中白细胞介素-1α（IL-1α）、白细胞介素-1β（IL-1β）、白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、干扰素-γ（IFNγ）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNFα）的检测频率是否更高。
采用酶联免疫吸附测定、放射免疫测定和生物测定技术，对78例硬皮病患者和73例对照者血清中的这些细胞因子浓度进行检测。
与对照组血清相比，硬皮病患者血清中IL-2、IL-4和IL-6的检测频率更高。患者血清和对照血清中TNFα和IL-1α的检出频率相同。在任何血清中均未检测到IL-1β和IFNγ。
IL-2、IL-4和IL-6可能是导致硬皮病患者发病过程的细胞因子之一。据我们所知，这是人类疾病中血清IL-4水平升高的首次报道。
