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黄独离体再生植株的遗传稳定性、茎尖包埋及高薯蓣皂苷元含量——薯蓣皂苷元的潜在替代来源

Genetic fidelity of in vitro regenerants, encapsulation of shoot tips and high diosgenin content in Dioscorea bulbifera L., a potential alternative source of diosgenin.

作者信息

Narula Alka, Kumar Sanjeev, Srivastava P S

机构信息

Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi, India.

出版信息

Biotechnol Lett. 2007 Apr;29(4):623-9. doi: 10.1007/s10529-006-9276-3. Epub 2007 Feb 22.

DOI:10.1007/s10529-006-9276-3
PMID:17318334
Abstract

Dioscorea bulbifera L. containing the pharmaceutically important compound, diosgenin, was regenerated in vitro through nodal segments on supplemented Murashige and Skoog medium (MS). Diosgenin was at 12 mg g(-1)dry wt in 12-week-old plantlets raised on MS with various growth hormones. Random amplified polymorphic DNA (RAPD) analysis showed genetic fidelity of regenerants. Encapsulation of shoot tips in 3% (w/v) calcium alginate for storage and germplasm exchange was achieved.

摘要

含有重要药用化合物薯蓣皂苷元的黄独通过在添加了Murashige和Skoog培养基（MS）的节段上进行离体再生。在添加了各种生长激素的MS培养基上培养12周的幼苗中，薯蓣皂苷元含量为12毫克/克干重。随机扩增多态性DNA（RAPD）分析表明再生植株具有遗传稳定性。实现了将茎尖包裹在3%（w/v）海藻酸钙中进行保存和种质交换。

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