Drewa Tomasz, Styczynski Jan
Department of Tissue Engineering, Chair of Medical Biology, Nicolaus Copernicus University, Karlowicza 24, 85-092, Bydgoszcz, Poland.
Int Urol Nephrol. 2007;39(3):851-7. doi: 10.1007/s11255-006-9105-6. Epub 2007 Feb 22.
The adult stem cells (ASC) are supposed to regenerate epithelium. We hypothesized prostate epithelial CD133-positive ASC to be responsible for establishing the primary cell culture. The prostate epithelial stem cells were isolated using anti-CD133 microbeads in order to form different cell populations. The morphology of cultures developed from CD133(+) and CD133(-) prostate epithelial cells were compared with prostate epithelium cell culture obtained after simple isolation procedure. Four 8-week-old Wistar rats were used in the experiment and six cultures were obtained. Double CD133(+) and CD133(-) cultures from two rats were established after enzymatic digestion and positive selection by SuperMACS device, and two non-selected CD133(+)/CD133(-) cultures were developed by simple prostate epithelial cell isolation from two other rats. The epithelial nature was confirmed by anti-cytokeratine antibodies. It was observed that growth of the CD133(+)/CD133(-) and CD133(+)culture resembled epithelial-like prostate cell culture. It was not possible to establish epithelial-like culture from CD133(-) cell population. The primary epithelial cell culture collapsed in a few days after the CD133-positive ASC were removed. We concluded that the epithelial progenitor cells are responsible for establishing primary prostate epithelial cultures in vitro.
成体干细胞（ASC）被认为可使上皮再生。我们推测前列腺上皮CD133阳性ASC负责建立原代细胞培养。使用抗CD133微珠分离前列腺上皮干细胞，以形成不同的细胞群体。将从CD133（+）和CD133（-）前列腺上皮细胞培养得到的细胞形态与经简单分离程序后获得的前列腺上皮细胞培养物进行比较。实验使用了4只8周龄的Wistar大鼠，获得了6种培养物。通过酶消化和SuperMACS装置进行阳性选择，建立了来自两只大鼠的双CD133（+）和CD133（-）培养物，另外两只大鼠通过简单的前列腺上皮细胞分离培养出两种未选择的CD133（+）/CD133（-）培养物。通过抗细胞角蛋白抗体证实了其上皮性质。观察到CD133（+）/CD133（-）和CD133（+）培养物的生长类似于上皮样前列腺细胞培养。无法从CD133（-）细胞群体建立上皮样培养物。去除CD133阳性ASC后，原代上皮细胞培养物在几天内就会崩溃。我们得出结论，上皮祖细胞负责在体外建立原代前列腺上皮培养物。
