Blasco Manuel, Badenes María Luisa, Del Mar Naval María
Fruit Tree Breeding Department, Instituto Valenciano de Investigaciones Agrarias (IVIA) , Apartado Oficial, 46113, Moncada, Valencia , Spain.
Breed Sci. 2016 Sep;66(4):606-612. doi: 10.1270/jsbbs.16021. Epub 2016 Aug 5.
Successful haploid induction in loquat ( (Thunb.) Lindl.) through in situ-induced parthenogenesis with gamma-ray irradiated pollen has been achieved. Female flowers of cultivar 'Algerie' were pollinated using pollen of cultivars 'Changhong-3', 'Cox' and 'Saval Brasil' irradiated with two doses of gamma rays, 150 and 300 Gy. The fruits were harvested 90, 105 and 120 days after pollination (dap). Four haploid plants were obtained from 'Algerie' pollinated with 300-Gy-treated pollen of 'Saval Brasil' from fruits harvested 105 dap. Haploidy was confirmed by flow cytometry and chromosome count. The haploids showed a very weak development compared to the diploid plants. This result suggests that irradiated pollen can be used to obtain parthenogenetic haploids.
通过用γ射线辐照花粉原位诱导孤雌生殖，已成功实现了枇杷（(Thunb.) Lindl.）的单倍体诱导。使用经150和300戈瑞两种剂量γ射线辐照的‘长虹3号’、‘考克斯’和‘萨瓦尔巴西’品种的花粉对‘阿尔及利亚’品种的雌花进行授粉。在授粉后90、105和120天收获果实。从授粉后105天收获的果实中，用经300戈瑞处理的‘萨瓦尔巴西’花粉授粉的‘阿尔及利亚’获得了4株单倍体植株。通过流式细胞术和染色体计数确认了单倍性。与二倍体植株相比，单倍体植株的发育非常弱。这一结果表明，辐照花粉可用于获得孤雌生殖单倍体。
