de Pater B S, Schilperoort R A
Department of Plant Molecular Biology, Leiden University, Netherlands.
Plant Mol Biol. 1992 Jan;18(1):161-4. doi: 10.1007/BF00018473.
Two rice cDNA clones (COS6 and COS9) were isolated, corresponding to genes that were highly expressed in roots from seedlings and mature plants. A genomic clone (GOS9) corresponding to cDNA clone COS9 was isolated and the intron/exon structure was determined by comparing the nucleotide sequences of the mRNA and the genomic clone. 5' ends and 3' ends of the mRNA were determined by primer extension and S1-nuclease mapping respectively. The open reading frame present in GOS9 potentially encodes a protein (14 kDa) that does not show any significant homology to other proteins in databases.
分离出两个水稻cDNA克隆（COS6和COS9），它们对应于在幼苗和成熟植株的根中高表达的基因。分离出了与cDNA克隆COS9对应的基因组克隆（GOS9），并通过比较mRNA和基因组克隆的核苷酸序列确定了内含子/外显子结构。分别通过引物延伸和S1核酸酶图谱分析确定了mRNA的5'端和3'端。GOS9中存在的开放阅读框可能编码一种蛋白质（14 kDa），该蛋白质与数据库中的其他蛋白质没有任何显著的同源性。
