Xie Y, Wu R
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853.
Gene. 1990 Mar 15;87(2):185-91. doi: 10.1016/0378-1119(90)90300-g.
An alcohol dehydrogenase-encoding gene was isolated from a rice genomic library. The coding region was sequenced, and the gene was identified as adh2. The nucleotide sequence of this gene contains an open reading frame which is interrupted by nine introns. Sequences that are important for eukaryotic gene expression, e.g. the TATA box, polyadenylation signal, intron/exon junction and putative splicing branch sites, are found in the expected locations in the rice adh2. A sequence related to the hexanucleotide core of regulatory elements identified in anaerobically induced plant genes is also present in the 5' region of the rice adh2.
从水稻基因组文库中分离出一个编码乙醇脱氢酶的基因。对编码区进行了测序，并将该基因鉴定为adh2。该基因的核苷酸序列包含一个开放阅读框，被9个内含子打断。对于真核基因表达重要的序列，如TATA框、聚腺苷酸化信号、内含子/外显子交界处和假定的剪接分支位点，在水稻adh2的预期位置被发现。在水稻adh2的5'区域也存在与厌氧诱导植物基因中鉴定的调控元件六核苷酸核心相关的序列。
