Matsumoto S, Oizumi N, Taira H, Ejiri S
Institute for Cell Biology and Genetics, Faculty of Agriculture, Iwate University, Morioka, Japan.
FEBS Lett. 1992 Oct 12;311(1):46-8. doi: 10.1016/0014-5793(92)81363-q.
A cDNA clone coding for rice elongation factor 1 beta' (EF-1 beta') was isolated from a rice anther cDNA library. The clone, named RB', was 980 bp long and contained a single open reading frame coding for 223 amino acids; the first 31 amino acids, except for the first methionine, which is absent in the mature protein, are identical to those of the purified protein determined with a protein sequencer. The amino acid sequence of rice EF-1 beta' shows homology to the C-terminal half of Artemia salina EF-1 beta (59%) and human EF-1 beta (63%), but might not have a phosphorylation site for casein kinase II which has been conserved in Artemia saline EF-1 beta and EF-1 delta, human EF-1 beta and silkworm EF-1 beta'.
从水稻花药cDNA文库中分离出一个编码水稻延伸因子1β'（EF-1β'）的cDNA克隆。该克隆名为RB'，长980 bp，包含一个编码223个氨基酸的单一开放阅读框；除成熟蛋白中不存在的第一个甲硫氨酸外，前31个氨基酸与用蛋白质测序仪测定的纯化蛋白的氨基酸相同。水稻EF-1β'的氨基酸序列与卤虫EF-1β的C端一半（59%）和人EF-1β（63%）具有同源性，但可能没有酪蛋白激酶II的磷酸化位点，该位点在卤虫EF-1β和EF-1δ、人EF-1β和家蚕EF-1β'中是保守的。
