Hanson R D, Ley T J
Department of Medicine, Jewish Hospital, Washington University Medical Center, St Louis, MO 63110.
Blood. 1992 Feb 1;79(3):610-8.
We have analyzed approximately 70 kb of the chromosome 14q11.2 hematopoietic serine protease gene cluster for the presence of nuclear scaffold attachment regions (SARs). At least 12 potential attachment sites were identified. SARs are present on both sides of the CGL-1/CSP-B and CGL-2/CCP-X genes and upstream from the cathepsin G (CG) gene. We have further characterized the SARs immediately flanking the cytotoxic lymphocyte-specific CGL-1/CSP-B gene. These 5' and 3' SARs are highly A-T-rich, contain multiple attachment sites, and are associated with the scaffolds of nuclei derived from both lymphoid and erythroid cell lines. These SARs contain multiple consensus elements frequently associated with A-T-rich sequences, including the vertebrate topoisomerase II (topo II) consensus sequence, the A-box and T-box elements, and the yeast autonomous replicating sequence (ARS). The potential role for the nuclear scaffold in the transcriptional regulation of CGL-1/CSP-B expression is discussed.
我们分析了14号染色体q11.2区域约70 kb的造血丝氨酸蛋白酶基因簇，以寻找核基质附着区域（SARs）。至少鉴定出12个潜在的附着位点。在CGL-1/CSP-B和CGL-2/CCP-X基因两侧以及组织蛋白酶G（CG）基因上游均存在SARs。我们进一步对细胞毒性淋巴细胞特异性CGL-1/CSP-B基因两侧紧邻的SARs进行了表征。这些5'和3' SARs富含A-T，包含多个附着位点，并与来源于淋巴细胞系和红细胞系的细胞核基质相关。这些SARs包含多个常与富含A-T序列相关的共有元件，包括脊椎动物拓扑异构酶II（topo II）共有序列、A盒和T盒元件以及酵母自主复制序列（ARS）。文中讨论了核基质在CGL-1/CSP-B表达转录调控中的潜在作用。
