Leculier C, Couprie N, Francina A, Archimbaud E, Adeleine P, Treille D, Denoyel G, Fiere D
Unité d'Immunologic, Institut Pasteur de Lyon, France.
Blood. 1992 Feb 1;79(3):760-4.
A murine monoclonal antibody against human lysozyme (AHL MoAb) was produced and tested on normal and leukemic monocytes using flow cytometry. The antibody gave a positive reactivity on normal monocytes permeabilized by saponin (82% to 98% of positive cells) and a negative reactivity on normal permeabilized neutrophils. This monocyte-specific reactivity had not been observed using a polyclonal antibody. Nevertheless, immunoblotting detected lysozyme in both monocyte and polymorphonuclear leukocyte (PMNL) lysates. The AHL MoAb, in the presence of lysozyme substrate (Micrococcus lysodeikticus cell walls), strongly inhibited the enzymatic activity. Flow cytometric analysis of leukemic cells isolated from patients suffering from different subtypes of acute myeloid leukemia (French-American-British [FAB] classification FAB M1-5) showed a highly significant positivity of FAB M5 for lysozyme compared with the other subtypes. The present results were consistent with the detection of a lysozyme epitope by AHL MoAb located near the catalytic site in monocytes. The same epitope was probably masked in PMNL granules.
制备了一种抗人溶菌酶的鼠单克隆抗体（AHL单克隆抗体），并使用流式细胞术对正常单核细胞和白血病单核细胞进行检测。该抗体对经皂角苷通透处理的正常单核细胞呈现阳性反应（阳性细胞占82%至98%），而对经通透处理的正常中性粒细胞呈现阴性反应。使用多克隆抗体未观察到这种单核细胞特异性反应。然而，免疫印迹法在单核细胞和多形核白细胞（PMNL）裂解物中均检测到了溶菌酶。在存在溶菌酶底物（溶壁微球菌细胞壁）的情况下，AHL单克隆抗体强烈抑制酶活性。对从患有不同亚型急性髓系白血病（法美英[FAB]分类FAB M1 - 5）患者中分离出的白血病细胞进行流式细胞术分析显示，与其他亚型相比，FAB M5的溶菌酶呈高度显著阳性。目前的结果与AHL单克隆抗体检测到位于单核细胞催化位点附近的溶菌酶表位一致。相同的表位可能在PMNL颗粒中被掩盖。
