Okano K, Yamamoto K, Ueda J, Fujii Y, Yonehara Y, Araki M
School of Allied Health Sciences, Yamaguchi University, Ube.
Rinsho Byori. 1994 Mar;42(3):278-82.
By means of the immunocytochemical method, the level of cytoplasmic lysozyme in leukocytes from healthy volunteers (n = 50) and from patients with uremia (n = 50), leukocytosis (n = 50), various forms of leukemia (n = 36) and myelodysplastic syndrome (MDS) (n = 7) were analysed, and compared with that of simultaneously assayed serum lysozyme. Both the cytoplasmic and serum levels of lysozyme in uremia and leukocytosis were significantly higher than normal subjects (p < 0.001). No correlation, however, was found between their cytoplasmic and serum levels of lysozyme. Morphological analysis for various kinds of leukemia and MDS indicated that myelocytic and monocytic cells became highly positive for lysozyme staining with maturation, and that lymphocytes, leukemic myeloblasts and monoblasts were negative. The cytoplasmic and serum lysozyme levels of leukemias or MDS having a number of lysozyme-positive cells were elevated as compared with those of normal individuals. Among them acute myelocytic leukemia (FAB M4) revealed an excellent correlation between the lysozyme levels in cytoplasm and in serum. The rest whose serum lysozyme level tend to be lower than the cytoplasmic one gave poor correlation. Thus, serum lysozyme level is not fully reflected by the cytoplasmic level. The dual determination of cytoplasmic and serum lysozyme is suggested to be helpful on estimating leukemia types, the degree of cellular maturation and total cell mass, and might also provide a valuable tool for prediction of prognosis for these disorders.
采用免疫细胞化学方法，分析了50名健康志愿者、50名尿毒症患者、50名白细胞增多症患者、36名各种类型白血病患者及7名骨髓增生异常综合征（MDS）患者白细胞中胞质溶菌酶水平，并与同时检测的血清溶菌酶水平进行比较。尿毒症和白细胞增多症患者的胞质和血清溶菌酶水平均显著高于正常受试者（p<0.001）。然而，其胞质和血清溶菌酶水平之间未发现相关性。对各种白血病和MDS的形态学分析表明，髓细胞和单核细胞随着成熟而溶菌酶染色呈高度阳性，而淋巴细胞、白血病原始粒细胞和原始单核细胞呈阴性。与正常个体相比，具有大量溶菌酶阳性细胞的白血病或MDS患者的胞质和血清溶菌酶水平升高。其中急性髓细胞白血病（FAB M4）的胞质和血清溶菌酶水平之间显示出良好的相关性。其余血清溶菌酶水平往往低于胞质溶菌酶水平的患者，相关性较差。因此，血清溶菌酶水平不能完全由胞质水平反映出来。建议同时测定胞质和血清溶菌酶有助于评估白血病类型、细胞成熟程度和细胞总量，也可能为预测这些疾病的预后提供有价值的工具。
