Caramelli David, Lalueza-Fox Carles, Capelli Cristian, Lari Martina, Sampietro María Lourdes, Gigli Elena, Milani Lucio, Pilli Elena, Guimaraes Silvia, Chiarelli Brunetto, Marin Vito Terribile Wien, Casoli Antonella, Stanyon Roscoe, Bertranpetit Jaume, Barbujani Guido
Dipartimento di Biologia Animale e Genetica, Laboratorio di Antropologia, Università di Firenze, via del Proconsolo 12, 50122 Firenze, Italy.
Forensic Sci Int. 2007 Nov 15;173(1):36-40. doi: 10.1016/j.forsciint.2007.01.020. Epub 2007 Feb 22.
We report on the mitochondrial DNA (mtDNA) analysis of the supposed remains of Francesco Petrarca exhumed in November 2003, from the S. Maria Assunta church, in Arquà Padua (Italy) where he died in 1374. The optimal preservation of the remains allowed the retrieval of sufficient mtDNA for genetic analysis. DNA was extracted from a rib and a tooth and mtDNA sequences were determined in multiple clones using the strictest criteria currently available for validation of ancient DNA sequences, including independent replication. MtDNA sequences from the tooth and rib were not identical, suggesting that they belonged to different individuals. Indeed, molecular gender determination showed that the postcranial remains belonged to a male while the skull belonged to a female. Historical records indicated that the remains were violated in 1630, possibly by thieves. These results are consistent with morphological investigations and confirm the importance of integrating molecular and morphological approaches in investigating historical remains.
我们报告了对2003年11月从意大利阿夸帕多瓦的圣玛丽亚阿松塔教堂挖掘出的弗朗切斯科·彼特拉克疑似遗骸进行的线粒体DNA（mtDNA）分析。彼特拉克于1374年在该教堂去世。遗骸保存状况极佳，得以获取足够的mtDNA用于基因分析。从一根肋骨和一颗牙齿中提取了DNA，并使用目前用于验证古代DNA序列的最严格标准（包括独立复制），在多个克隆中确定了mtDNA序列。来自牙齿和肋骨的mtDNA序列并不相同，这表明它们属于不同个体。实际上，分子性别鉴定显示，颅后遗骸属于男性，而头骨属于女性。历史记录表明，这些遗骸在1630年可能被盗墓者破坏。这些结果与形态学研究结果一致，并证实了在研究历史遗骸时整合分子和形态学方法的重要性。
