Xu Zhi, Zhang Fan, Xu Bosong, Tan Jingze, Li Shilin, Li Chunxiang, Zhou Hui, Zhu Hong, Zhang Jun, Duan Qingbo, Jin Li
Ministry of Education (MOE) Key Laboratory of Contemporary Anthropology and Center for Evolutionary Biology, School of Life Sciences and Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
PLoS One. 2008 Oct 1;3(10):e3275. doi: 10.1371/journal.pone.0003275.
Variant studies on ancient DNA have attempted to reveal individual origin. Here, based on cloning sequencing and polymerase chain reaction-restriction fragment length polymorphisms, we analyzed polymorphisms in the first hypervariable region and coding regions of mitochondrial DNA of 19 human bone remains which were excavated from a tomb near the Terra Cotta Warriors and dated some 2,200 years before present. With the aim of shedding light on origins of these samples who were supposed to be workers building the mausoleum for the First Emperor of China, we compared them with 2,164 mtDNA profiles from 32 contemporary Chinese populations at both population and individual levels. Our results showed that mausoleum-building workers may be derived from very diverse sources of origin.
古代DNA的变异研究试图揭示个体起源。在此，基于克隆测序和聚合酶链反应-限制性片段长度多态性，我们分析了从秦始皇兵马俑附近一座古墓中发掘出的19具人类骨骼遗骸线粒体DNA第一高变区和编码区的多态性，这些遗骸的年代约为距今2200年前。为了弄清楚这些被认为是为中国始皇帝修建陵墓的工人的起源，我们在群体和个体层面将他们与来自32个当代中国群体的2164个线粒体DNA图谱进行了比较。我们的结果表明，修建陵墓的工人可能来自非常多样化的起源地。
