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用于收集接触性证据的双拭子技术。

Double swab technique for collecting touched evidence.

作者信息

Pang B C M, Cheung B K K

机构信息

Forensic Science Division, Hong Kong Government Laboratory, Homantin Government Offices, 88 Chung Hau Street, Kowloon, Hong Kong SAR, China.

出版信息

Leg Med (Tokyo). 2007 Jul;9(4):181-4. doi: 10.1016/j.legalmed.2006.12.003. Epub 2007 Feb 22.

Abstract

Touched evidence is often submitted to forensic laboratories for DNA analysis. Classical stain recovery technique, involving one wet cotton swab, is commonly used for recovering the touched evidence. Double swab technique, using a wet cotton swab followed by a dry cotton swab, was compared with the classical technique for recovering the touched evidence. The wet cotton swabs and the dry cotton swabs were individually extracted. DNA extracts were quantified and amplified at 15 polymorphic loci. DNA recovered in some of the second dry swabs contained sufficient amount of DNA to yield a DNA profile. This study shows that the double swab technique improves the quality of the resulting DNA profiles. The double swab technique for recovering touched evidence at crime scenes is recommended.

摘要

接触痕迹证据通常会被提交到法医实验室进行DNA分析。传统的污渍提取技术,即使用一根湿棉签,常用于提取接触痕迹证据。双棉签技术,即先用一根湿棉签,再用一根干棉签,与传统技术在提取接触痕迹证据方面进行了比较。湿棉签和干棉签分别进行提取。对DNA提取物进行定量,并在15个多态性位点进行扩增。一些第二根干棉签中回收的DNA含有足够的量以产生DNA图谱。本研究表明,双棉签技术提高了所得DNA图谱的质量。建议采用双棉签技术在犯罪现场提取接触痕迹证据。

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