Netherlands Forensic Institute, P.O. Box 24044, 2490 AA The Hague, Netherlands.
Forensic Sci Int Genet. 2012 Mar;6(2):219-23. doi: 10.1016/j.fsigen.2011.04.019. Epub 2011 May 24.
After manual strangulation, epithelial cells originating from the offender can often be found on the skin of the victim. In order to obtain a conclusive DNA profile, it is important to secure as many epithelial cells from the offender and as few epithelial cells from the victim as possible. In this study, two methods for securing offender DNA were compared: the double swab method and an adapted tape-lifting method, so-called stubbing. 50 male volunteers were asked to simulate manual strangulation on the forearm of a female volunteer. After securing the epithelial material, DNA profiles were generated. The contribution of both donors to the samples was determined from the number of detected alleles, specific for each donor, and the average peak height of the donor-specific alleles. For the offender, in all cases except one, partial or full profiles were obtained and no difference between the double swab and the stubbing method was observed. For the victim, fewer alleles were detected by means of double swab than by means of stubbing. In conclusion, the double swab method performs slightly better than the stubbing method. However, from a practical point of view, the stubbing method may be preferred over the double swab technique.
在手动勒颈后，通常可以在受害者的皮肤上发现来自施害者的上皮细胞。为了获得明确的 DNA 图谱，重要的是尽可能多地从施害者身上获取上皮细胞，同时尽可能少地从受害者身上获取上皮细胞。在这项研究中，比较了两种获取施害者 DNA 的方法：双拭子法和改良的胶带提取法，即所谓的 Stubbing。50 名男性志愿者被要求在一名女性志愿者的前臂上模拟手动勒颈。在获取上皮材料后，生成了 DNA 图谱。从每个供体特有的、被检测到的等位基因的数量以及供体特异性等位基因的平均峰高，确定了两个供体对样本的贡献。对于施害者，除了一个案例外，在所有其他案例中都获得了部分或完整的图谱，而且没有观察到双拭子法和 Stubbing 法之间的差异。对于受害者，通过双拭子法检测到的等位基因比通过 Stubbing 法少。总之，双拭子法的表现略优于 Stubbing 法。然而，从实际角度来看，Stubbing 法可能比双拭子技术更受欢迎。
