Lim P O, Sears B B, Klomparens K L
Genetics Program, Michigan State University, East Lansing 48824.
J Bacteriol. 1992 Feb;174(3):682-6. doi: 10.1128/jb.174.3.682-686.1992.
In terms of biosystematics, the plant-pathogenic mycoplasmalike organisms (MLOs) have been tentatively placed into the class Mollicutes. Certain physiological tests have been used to distinguish families within this class: the sterol-nonrequiring Acholeplasmataceae differ from the sterol-requiring Mycoplasmataceae in that the former are more resistant to lysis by digitonin and more sensitive to lysis in hypotonic salt solutions. To test MLOs for these membrane properties and thus assist in their definitive classification, a dot-blot microassay procedure was used to detect nucleic acids released from lysed cells. The results show that MLOs resemble acholeplasmas grown in the absence of sterols in that they are resistant to digitonin and sensitive to hypotonic salt solutions. The MLOs can be differentiated from acholeplasmas grown without sterols by their greater resistance to lysis in hypotonic sucrose solutions.
在生物系统学方面，植物致病性类支原体生物（MLOs）已被初步归入柔膜菌纲。某些生理测试已被用于区分该纲内的科：不需要固醇的无胆甾原体科不同于需要固醇的支原体科，前者对洋地黄皂苷的裂解更具抗性，而在低渗盐溶液中对裂解更敏感。为了测试MLOs的这些膜特性，从而辅助它们的明确分类，采用斑点印迹微量测定法来检测裂解细胞释放的核酸。结果表明，MLOs类似于在无固醇条件下生长的无胆甾原体，因为它们对洋地黄皂苷具有抗性，且对低渗盐溶液敏感。MLOs与在无固醇条件下生长的无胆甾原体的区别在于，它们在低渗蔗糖溶液中对裂解具有更强的抗性。
