Yee T, Inouye M
J Bacteriol. 1981 Mar;145(3):1257-65. doi: 10.1128/jb.145.3.1257-1265.1981.
The genome sizes of two myxobacteria, Myxococcus xanthus and Stigmatella aurantiaca, were measured by renaturation analysis and also by a new method involving the quantitation of individual restriction fragments. In contrast to several previous reports, which indicate that M. xanthus has a genome size which is three to four times that of Escherichia coli, the present measurements indicated that the M. xanthus genome is only about 24 to 53% larger than that of E. coli. S. aurantiaca had a genome size nearly identical to that of M. xanthus. Of possible significance is the fact that the renaturation curves of M. xanthus and S. aurantiaca deoxyribonucleic acid both exhibited significant fractions which renatured with rapid, unimolecular kinetics. However, we were unable to establish that these fractions represented inverted repeats of repetitive sequences.
通过复性分析以及一种涉及对单个限制性片段进行定量的新方法，测量了两种黏细菌——黄色黏球菌和橙色标桩菌的基因组大小。与之前的几份报告相反，之前的报告表明黄色黏球菌的基因组大小是大肠杆菌的三到四倍，而目前的测量结果表明，黄色黏球菌的基因组仅比大肠杆菌大约大24%至53%。橙色标桩菌的基因组大小与黄色黏球菌几乎相同。一个可能具有重要意义的事实是，黄色黏球菌和橙色标桩菌的脱氧核糖核酸的复性曲线都显示出相当大的部分以快速的单分子动力学进行复性。然而，我们无法确定这些部分代表重复序列的反向重复。
